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Topo Kits

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TG4037
Is used to terminate topoisomerase reactions.  To terminate reactions, add 1/5 volume to the reaction prior to loading agarose gel.      
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TG4030
E. coli DNA gyrase assay buffer supplied as a 5X stock solution.  The buffer is optimized and tested for E. coli DNA gyrase only.      
$95.00
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TG4055
5X Potassium Glutamate (1ml).
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TG4039
Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times.  The activity of proteinase K is increased at…
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Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times.  The activity of proteinase K is increased at elevated temperatures up to 65° C. Calcium […]
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$95.00
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TG4045
S. Aureus Gyrase Assay Buffer (1ml).
$95.00
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TG4095
Topo I Dilution Buffer is supplied as a working solution at 1X solution.  The buffer is optimized and tested for use as a diluent for recombinant human topoisomerase I enzyme from TopoGEN.
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TG4040
Topo II Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use: 10X Incomplete Topo II Assay Buffer A contains the following: 0.5M Tris-HCl (pH8.0), 1.5M NaCl, 0.1M MgCl2, 5 mM dithiothreitol. 10X ATP Buffer B contains 20…
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Topo II Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use: 10X Incomplete Topo II Assay Buffer A contains the following: 0.5M Tris-HCl (pH8.0), 1.5M NaCl, 0.1M MgCl2, 5 mM dithiothreitol. 10X ATP Buffer B contains 20 mM ATP in water. These two buffers must be […]
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$105.00
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TG4090
Topo II Dilution Buffer is supplied as a working solution at 1X solution.  The buffer is optimized and tested for use as a diluent for TopoGEN’s type II topoisomerase.        
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TG4032
Topo IV Assay Buffer is supplied as a 5x Stock solution in two parts that must be mixed prior to use: These two buffers must be mixed to prepare a final 5x complete Topo IV Assay Buffer (A+B) as follows: add 0.1 volume of Buffer B to 1.0…
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Topo IV Assay Buffer is supplied as a 5x Stock solution in two parts that must be mixed prior to use: These two buffers must be mixed to prepare a final 5x complete Topo IV Assay Buffer (A+B) as follows: add 0.1 volume of Buffer B to 1.0 volume Buffer A. Prepare only as much […]
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