Hycamtin (topotecan hydrochloride) is a semi-synthetic derivative of camptothecin and is an anti-tumor drug that targets topo I and is a topo I poison (induces cleavage complexes).
Chemical Name: (S)-10-[(dimethylamino) methyl]-4-ethyl-4,9-dihydroxy-1H- pyrano[3′, 4′: 6,7] indolizino [1,2-b] quinoline-3,14-(4H,12H)-dione monohydrochloride
Molecular Formula: C23H23N3O5•HCl
Molecular Weight: 457.9
Topotecan hydrochloride is soluble in water and melts with decomposition at 213° to 218°C. TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages). Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.
TG4100 is supplied already suspended in water at 10 mM (0.25 ml) and is ready for use. The drug is pre-tested and shown to induce topo cleavages using purified human topoisomerase from TopoGEN.
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