The Western blot marker set for topo I is used to unambiguously locate the position of the 100 kDa form of intact topo I. This product is supplied in SDS-PAGE loading buffer and ready to load directly onto an SDS-PAGE.Description of Product
20 units of topo I marker (at 0.1 units/ul) in lx SDS-PAGE load buffer. Total volume is 200 ul.
The marker is shipped on blue ice and should be stored at -20°C.
- The presence of the 100 kDa band has been confirmed by Western blotting.
- There are no extraneous polypeptides in this preparation.
- Loading 10 ul of extract is sufficient for use as a marker in Western blotting.