The TopI-NucEx Kit is designed to allow a researcher to prepare a nuclear extract enriched for type I topoisomerase (topo I) activity and intact 100 kDa polypeptide. The appropriate assay buffer specific for topo I is included. This kit is suitable for most animal cell lines growing in vitro but is optimized for human and rodent species. The TopI-NucEx Kit also allows the investigator to quantify topoisomerase I activity in any given cell line and provides the end user a source of enzyme for preliminary drug screening or high throughput screening (HTS).
Note that this kit contains everything needed to assay topo I in a crude extract (including plasmid DNA, buffers and markers). The kit is suitable for drug screening experiments. Additional materials for the assay should be ordered to replenish the kit, including DNA substrate and marker DNA, as well as control drugs, such as Camptothecin or etoposide if you are performing drug screening experiments.