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Product ID | Product Info | Description |
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DR3000 |
DNA Repair pathways in animal cells can be divided into two main categories: HR and NHEJ. HR or homologous recombination is a minor pathway but very important in protecting cells from genotoxicity. The process has two key requirements as well: a homologous sequence, usually available after DNA replication… Show more (+) DNA Repair pathways in animal cells can be divided into two main categories: HR and NHEJ. HR or homologous recombination is a minor pathway but very important in protecting cells from genotoxicity. The process has two key requirements as well: a homologous sequence, usually available after DNA replication when the genome is 4N, and S-phase. […] Show less (-) |
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DR3000-HRCN1.4-Sce |
This is the first HR reporter that has been engineered into a neuronally derived mouse cell line model. The CN1.4 cell line is a clone derived from mouse embryonic day 13 neuronal cultures. The line was transformed using the temperature-sensitive SV-40 Large T-antigen. The cell line expresses… Show more (+) This is the first HR reporter that has been engineered into a neuronally derived mouse cell line model. The CN1.4 cell line is a clone derived from mouse embryonic day 13 neuronal cultures. The line was transformed using the temperature-sensitive SV-40 Large T-antigen. The cell line expresses numerous neuronal markers, including neurofilaments, glutamic acid decarboxylase […] Show less (-) |
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DR5000 |
Custom Cell Based Screening Kit for NHEJ contains reagents necessary to create a complete NHEJ reporter system in any animal cell line. It includes the NHEJ-GFP reporter with a selectable marker and a DS DNA cleavage nuclease to initiate DNA repair. |
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DR5001-HNHeLa-Sce |
This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and other genes) that affect or impact NHEJ repair. The kit uses GFP as an in situ readout for NHEJ activity in a cell context. |
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DR3000-HRHeLa-Sce |
This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in situ readout for the HR pathway. |
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DR3050 |
This is a custom cell-based reporter system designed to allow researchers to study homologous recombination (HR) in a cell line of their choosing. The kit uses GFP as an in vivo readout for the HR pathway and includes proper controls to set the system up in your own lab. Because… Show more (+) This is a custom cell-based reporter system designed to allow researchers to study homologous recombination (HR) in a cell line of their choosing. The kit uses GFP as an in vivo readout for the HR pathway and includes proper controls to set the system up in your own lab. Because this system relies on pools of cells that take up reporter and effector genes (HR-GFP and I-Sce1 respectively) the system can be ‘hosted’ in any cell of interest. The only limit is transfection efficiency. There is no need to engage in time consuming and labor intensive cell line cloning and development...you simply transfect and let the system proceed through HR over a 48h period. Easy! Show less (-) |
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DR5000-HNSH-Sce |
This is a cell-based reporter kit that is hosted by a human neuroblastoma cell line (SH-SY5Y) that can grow continuously in vitro and will terminally differentiate. This provides the opportunity of analyzing NHEJ in growing or terminally differentiated cells in a neuronal lineage. |
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DR3000-HRHeLa |
This is a cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in vivo readout for the HR pathway and Crispr-CAS… Show more (+) This is a cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in vivo readout for the HR pathway and Crispr-CAS gRNA plasmid that drives a […] Show less (-) |
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DR5000-HN3T3-Sce |
This is a Mouse 3T3 cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and other genes) that affect or impact NHEJ repair. The kit uses GFP as an in situ readout for NHEJ activity in a cell context… Show more (+) This is a Mouse 3T3 cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and other genes) that affect or impact NHEJ repair. The kit uses GFP as an in situ readout for NHEJ activity in a cell context. Show less (-) |
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DR3000-HRSH-Sce |
DNA Repair pathways in animal cells can be divided into two main categories: HR and NHEJ. HR or homologous recombination is a minor pathway but very important in protecting cells from genotoxicity. HR needs a homologous sequence and is limited to S-phase. A specific reporter based assay for HR… Show more (+) DNA Repair pathways in animal cells can be divided into two main categories: HR and NHEJ. HR or homologous recombination is a minor pathway but very important in protecting cells from genotoxicity. HR needs a homologous sequence and is limited to S-phase. A specific reporter based assay for HR can be very beneficial for drug […] Show less (-) |
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DR5000-HNCN1.4 |
TopoGEN scientists have created a novel, cell based kit to follow NHEJ by simply assaying for the presence of GFP positive cells (Fig 1). DNA Repair pathways in animal cells can be divided into two main categories: HR and NHEJ. Homologous recombination (HR) is a minor pathway but very… Show more (+) TopoGEN scientists have created a novel, cell based kit to follow NHEJ by simply assaying for the presence of GFP positive cells (Fig 1). DNA Repair pathways in animal cells can be divided into two main categories: HR and NHEJ. Homologous recombination (HR) is a minor pathway but very important in protecting cells from genotoxicity. […] Show less (-) |
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DR3000-HR3T3-Sce |
This is a cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in vivo readout for the HR pathway and a… Show more (+) This is a cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in vivo readout for the HR pathway and a plasmid expression vector containing the […] Show less (-) |
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TG2000G |
Highly purified E. coli DNA Gyrase is offered as a holoenzyme (contains both A,B subunits). This is an excellent reagent for supercoiling plasmids in vitro or for novel drug screens. |
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TG2000GSA |
Highly purified S. aureus DNA gyrase is a type II topoisomerase encoded by two genes GyrA and GyrB from staphylococcus aureus. DNA gyrase is an essential topoisomerase that supercoils DNA through a process of strand breakage/resealing and DNA wrapping. As a type II enzyme, gyrase is unique in… Show more (+) Highly purified S. aureus DNA gyrase is a type II topoisomerase encoded by two genes GyrA and GyrB from staphylococcus aureus. DNA gyrase is an essential topoisomerase that supercoils DNA through a process of strand breakage/resealing and DNA wrapping. As a type II enzyme, gyrase is unique in its ability to negatively supercoil a relaxed […] Show less (-) |
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TG2035 |
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Relaxed pHOT-1 Plasmid DNA is the ideal substrate for use assaying the supercoiling activity of DNA gyrase.
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TG2020-1 |
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can… Show more (+) Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can be avoided by using a catenated DNA […] Show less (-) |
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TG2018-1 |
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can… Show more (+) Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can be avoided by using a catenated DNA […] Show less (-) |
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TG2013 |
Kinetoplast DNA (kDNA) is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I. Thus, kDNA works well to quantify type II activity in… Show more (+) Kinetoplast DNA (kDNA) is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I. Thus, kDNA works well to quantify type II activity in crude cell extracts, which are frequently overloaded […] Show less (-) |
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TG2030 |
This plasmid is a perfect substrate for assaying any topoisomerase. pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers. Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase. Supercoiled pHOT-1 is… Show more (+) This plasmid is a perfect substrate for assaying any topoisomerase. pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers. Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase. Supercoiled pHOT-1 is a scaled-down derivative of pBR322 and optimized for higher […] Show less (-) |
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TG2001GKIT |
This kit contains purified bacterial (E. coli) DNA Gyrase purified to homogeneity (>98% based on SDS-PAGE). The Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex. The enzyme is supplied at the unit concentration given on the quality control data provided… Show more (+) This kit contains purified bacterial (E. coli) DNA Gyrase purified to homogeneity (>98% based on SDS-PAGE). The Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex. The enzyme is supplied at the unit concentration given on the quality control data provided with the kit. It is also stored […] Show less (-) |
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When you become our customer, we become your partner in research. We review your data and offer valuable input. Feel free to contact us for further information about how we can assist your topoisomerase research endeavors.