Genistein is an isoflavone and an inhibitor of tyrosine protein kinases. It has been reported to induce topo II dependent cleavages (Biochem. Pharmacol. 39:737-740 ). TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II (Note, Genistein may also inhibit topoisomerase I.) For in vitro cleavage detection, we recom- mend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages). Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.
SKU: TG4160 Categories: Topo II, Topoisomerase Related Drugs Tag: genistein
Chemical Name: 5,7-Dihydroxy-3-(4-hydroxyphenyl)-4H-1- benzopyran-4-one
Molecular Formula: C15H10O5
Molecular Weight: 270.23
TG4160 is supplied already suspended in DMSO at 10 mM (0.25 ml) and is ready for use. The drug is pre-tested and shown to induce topo cleavages using purified human topoisomerase from TopoGEN.
The liquid product is shipped on blue ice. Store at -20°C.
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