all of OUR PRODUCTS are validated & certified by TopoGEN Scientists
DNA Substrates
Filters
Sort results
Reset
Apply
Product ID | Product Info | Description | Purchase |
|---|---|---|---|
TG2020-1 |
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can… Read more (+) Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can be avoided by using a catenated DNA […] Show less (-) |
||
TG2013 |
Kinetoplast DNA (kDNA) is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I. Thus, kDNA works well to quantify type II activity in… Read more (+) Kinetoplast DNA (kDNA) is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I. Thus, kDNA works well to quantify type II activity in crude cell extracts, which are frequently overloaded […] Show less (-) |
||
TG2018-1 |
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can… Read more (+) Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can be avoided by using a catenated DNA […] Show less (-) |
||
TG2035 |
Read more (+)
Relaxed pHOT-1 Plasmid DNA is the ideal substrate for use assaying the supercoiling activity of DNA gyrase.
Show less (-) |
||
TG2030 |
This plasmid is a perfect substrate for assaying any topoisomerase. pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers. Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase. Supercoiled pHOT-1 is… Read more (+) This plasmid is a perfect substrate for assaying any topoisomerase. pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers. Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase. Supercoiled pHOT-1 is a scaled-down derivative of pBR322 and optimized for higher […] Show less (-) |
OUR SERVICES
Why Choose Us
When you become our customer, we become your partner in research. We review your data and offer valuable input. Feel free to contact us for further information about how we can assist your topoisomerase research endeavors.