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Custom Screening Services and Contract R&D

TopoGEN offers turn-key, custom screening services to screen for new Topo Drugs that target Human Topo I, Topo II, Topo IV and DNA Gyrase. As an Experienced CRO, the advantages are numerous! Our group has >30 years experience as a Contract Research Organization. We guarantee clear, cosmetic data, unambiguous interpretations, and that you will receive publication quality results. Since we are seasoned veterans in the arena of in vitro testing, we can help you develop a tractable strategy for analysis of drug mechanisms in the context of the tumor cell. We can evaluate potential genotoxicity independently from topo effects without extra charge.

All our topo enzymes are homogeneously pure. We do not screen using extracts. Controls with specific drugs (e.g. etoposide vs. Top2a) guarantee specificity in all assays and validate even negative results with unknowns.

Additionally, we produce quality topoisomerases & related assay and drug screening kits as technology transfer platforms from our lab to yours. When you become our customer, we become your partner in research. We review your data and offer valuable input. Feel free to contact us for further information about how we can assist your topoisomerase research endeavors.

CLICK HERE TO FILL OUT OUR CUSTOM QUOTE FORM

HUMAN TOPO SCREENING IN VITRO

Do have a drug that might target human topoisomerases?

We can help! Let us design a tractable screen to validate your novel small molecule or natural product. We do this routinely for researchers who want high quality, unambiguous results in a short time frame. Go HERE to fill out a screening request and we can generate a quote. Projects run (typically) for a few business days to a few weeks depending on number of compounds being screened. We cover all the bases: we can test multiple enzymes for a comprehensive result and, we’ll determine the mechanism of action of your novel drug.

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BACTERIAL GYRASE & TOPO IV SCREENING

Do have a drug that might target bacterial DNA Gyrase or Topo IV? Also, you may need to test whether your new antibiotic drug crosses over and alters the human enzyme, Top2.

We can help! Let us design a tractable screen to validate and test for antibiotic activity. Everyone knows that DNA Gyrase is an excellent target protein for new antibiotics. You may have the next big bug buster! We do this routinely for researchers who want high quality, unambiguous results in a short time frame. Go HERE to fill out a screening request and we can generate a quote. Projects run (typically) for a few business days to a few weeks depending on number of compounds being screened. We cover all the bases: we can test multiple enzymes for a comprehensive result and, we’ll determine the mechanism of action of your new antibiotic.

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CELL CONTEXT TOPOISOMERASE SCREENING (ICE ASSAYS)

A key piece in drug discovery is actually showing that a new drug works in live cells. We can do this for you (in a cell line of your choosing) or you can purchase our ICE Assay Kit (Cat #TG1020) for your own lab. ICE stands for “In vivo Complex of Enzyme” and is based on the fact that endogenous topoisomerases become covalently bound to genomic DNA in the presence of drugs that ‘poison’ the reaction (leading to DNA damage). The ICE Assay simply measures the covalent adduct formation in a cell context. Good Topo poisons, like etoposide and camptothecin, will induce Top2 or Top1, respectively, to bind irreversibly to cellular DNA. The ICE Assay measures the amount of Top2 or Top1 that is bound. The assay is not difficult but is technically complicated.

If you want it done quickly and clearly, let us help. We have a number of different cell lines (please enquire) or you can send us your cells; either way we’re here to help. Note that our ICE Assay Kits are based on specific antibodies, so you know what topo isoform you are targeting!

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BIO-CORRELATIONS IN CLINICIAL TRIALS

We can assist in clinical trials by measuring Topo levels or using ICE to examine Topo/DNA complexes. These are good biomarkers for clinical trials. Let us know how we can help.

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WESTERN BLOTTING

TopoGEN can quantify topoisomerase I, IIa, IIb and III levels in your extracts using Western Blotting and utilize our immune-reagents for cell distribution or localization experiments. We can also provide precise directions on making nuclear extracts and we will quantify topo levels by high resolution Western Blotting. The data will be publication quality with markers and precise quantitation. Note that we can also help you optimize your detection of topo protein levels using Western Blotting.

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QUANTIFY TOPOISOMERASE ACTIVITY LEVELS IN CRUDE EXTRACTS

We can quantify total topoisomerase I, II activity levels in your specific model cell line or help you prepare nuclear extracts. We will quantify topo levels using biochemical assays (relaxation, decatenation or even Westerns for total topo protein levels). The data will be publication quality with markers and precise quantitation. This is very useful in a wide variety of applications. Typically, the customer is instructed on a defined protocol to make the extract and submits the samples for testing. We carry out titrations, quantify band intensities and analyze the data.

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TOPOISOMERASE GENOMIC DNA COMPLEXES

Interested in a bioinformatic analysis of covalent Top2 or Top1/genomic DNA complexes isolated from chromatin in any given cell line? We can help. We use validated “Topo-CHIP” technologies that rely on anti-topoisomerase antibodies to immunoprecipitated the attached DNA.