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Topo Kits

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Topo Kits
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TG1001
This kit is designed to allow quick and specific assays for eukaryotic type II DNA topoisomerases. This kit facilitates the purification and characterization of type II enzymes and contains all reagents necessary for routine assays of type II topoisomerases. The assay is based upon decatentation of kinetoplast DNA (kDNA) and…
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This kit is designed to allow quick and specific assays for eukaryotic type II DNA topoisomerases. This kit facilitates the purification and characterization of type II enzymes and contains all reagents necessary for routine assays of type II topoisomerases. The assay is based upon decatentation of kinetoplast DNA (kDNA) and because it is specific for […]
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TG1015
This kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in…
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This kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking […]
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TG1003
The Kit is designed to allow quick and specific detection of DNA gyrase. This kit facilitates the purification and characterization of type II topoisomerase enzymes (DNA Gyrase) and contains all reagents necessary for routine assays of type II enzymes that either have or do not have the ability to supercoil…
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The Kit is designed to allow quick and specific detection of DNA gyrase. This kit facilitates the purification and characterization of type II topoisomerase enzymes (DNA Gyrase) and contains all reagents necessary for routine assays of type II enzymes that either have or do not have the ability to supercoil.      
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295.00
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TG1025
The purpose of the DNA Intercalator/Unwinding Kit is to determine if a compound induces DNA unwinding characteristic of DNA intercalators. Sufficient materials are provided for at least 50 independent determinations. NOTE: enzyme is not included with this kit.  
465.00
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TG2000GKIT
The E. coli DNA Gyrase and Relaxed DNA Assay Kit  is designed to provide the investigator quick and specific assays for DNA gyrase.  These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work.  These kits are also appropriate for the…
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The E. coli DNA Gyrase and Relaxed DNA Assay Kit  is designed to provide the investigator quick and specific assays for DNA gyrase.  These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work.  These kits are also appropriate for the researcher who is interested in large scale […]
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TG1040-1
This Kit is designed to allow a researcher to prepare a nuclear extract enriched for type I topoisomerase (topo I) activity and intact 100 kDa polypeptide.  The appropriate assay buffer specific for topo I is included.  This kit is suitable for most animal cell lines growing in vitro…
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This Kit is designed to allow a researcher to prepare a nuclear extract enriched for type I topoisomerase (topo I) activity and intact 100 kDa polypeptide.  The appropriate assay buffer specific for topo I is included.  This kit is suitable for most animal cell lines growing in vitro but is optimized for human and rodent […]
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405.00
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TG1050-1
This Kit is designed to allow a researcher to prepare a nuclear extract enriched for type II topoisomerase (topo II) activity.  The appropriate assay buffer specific for topo II is included.  This kit is suitable for most animal cell lines growing in vitro but is optimized for human…
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This Kit is designed to allow a researcher to prepare a nuclear extract enriched for type II topoisomerase (topo II) activity.  The appropriate assay buffer specific for topo II is included.  This kit is suitable for most animal cell lines growing in vitro but is optimized for human and rodent species. This Kit also allows […]
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410.00
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TG1020
TopoGEN has extensive experience with cell-based screens for topoisomerase targeting.  Our staff scientists developed and published a method for detecting covalent topo/genomic DNA complexes in live cells (1,2) that is widely utilized in the field.  The method, called the ICE Assay (In vivo Complex of Enzyme) originally…
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TopoGEN has extensive experience with cell-based screens for topoisomerase targeting.  Our staff scientists developed and published a method for detecting covalent topo/genomic DNA complexes in live cells (1,2) that is widely utilized in the field.  The method, called the ICE Assay (In vivo Complex of Enzyme) originally required a CsCl gradient to purify genomic DNA […]
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TG4060
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).  The ability of each lot to function as an effective topoisomerase denaturant is tested and verified for each lot.  
95.00
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TG4038
Vial contains 1ml of 10X agarose gel loading buffer ( 0.005% bromophenol blue, 50% glycerol).    
95.00
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TG4065
20% Sarkosyl
95.00
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TG4035
20mM ATP supplied in aqueous buffer (0.5ml).
95.00
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TG4037
Is used to terminate topoisomerase reactions.  To terminate reactions, add 1/5 volume to the reaction prior to loading agarose gel.      
95.00
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TG4030
E. coli DNA gyrase assay buffer supplied as a 5X stock solution.  The buffer is optimized and tested for E. coli DNA gyrase only.      
95.00
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TG4055
5X Potassium Glutamate (1ml).
95.00
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