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Topo Kits

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TG1001
This kit is designed to allow quick and specific assays for any type II topoisomerase, either eu- or prokaryotic. This kit facilitates the purification and characterization of type II enzymes and contains all reagents necessary for routine assays of Topoisomerase II.
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TG1015
The Human Topoisomerase I Assay kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction…
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The Human Topoisomerase I Assay kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order […]
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TG1030
This assay kit contains all the reagents for cytolocalization of Human Top2a using immunofluorescence.  The system is optimized for use with FITC or Rhodamine labeled secondary antibody with adherent cells.  The method may work with other cells besides human. Mouse, rat and monkey cells will cross react with…
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This assay kit contains all the reagents for cytolocalization of Human Top2a using immunofluorescence.  The system is optimized for use with FITC or Rhodamine labeled secondary antibody with adherent cells.  The method may work with other cells besides human. Mouse, rat and monkey cells will cross react with topo II antibody.  The antibody is specific […]
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$485.00
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TG1003
The Kit is designed to allow quick and specific detection of DNA gyrase. This kit facilitates the purification and characterization of type II topoisomerase enzymes (DNA Gyrase) and contains all reagents necessary for routine assays of type II enzymes that either have or do not have the ability to supercoil…
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The Kit is designed to allow quick and specific detection of DNA gyrase. This kit facilitates the purification and characterization of type II topoisomerase enzymes (DNA Gyrase) and contains all reagents necessary for routine assays of type II enzymes that either have or do not have the ability to supercoil.      
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$295.00
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TG1035
The immunoprecpitation (IP) kit contains the key reagents and protocol for immunoprecipitating Human Top2a.  The method should work well with other cells besides humans.  For example, mouse, rat and monkey cells will cross react with topoisomerase II antibody.  The antibody is specific for p170 (topo II alpha)…
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The immunoprecpitation (IP) kit contains the key reagents and protocol for immunoprecipitating Human Top2a.  The method should work well with other cells besides humans.  For example, mouse, rat and monkey cells will cross react with topoisomerase II antibody.  The antibody is specific for p170 (topo II alpha) in human (HeLa) cells and although we presume […]
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$485.00
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TG1025
The purpose of the DNA Intercalator/Unwinding Kit is to determine if a compound induces DNA unwinding characteristic of DNA intercalators. Sufficient materials are provided for at least 50 independent determinations.  NOTE: enzyme is not included with this kit.  
$465.00
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TG1024
TopoGEN has extensive experience with assays for topoisomerase inhibition in vivo. This analysis allows the investigator to ascertain whether a novel agent is active against endogenous Top2bin whole cells. An important benefit to this analysis is that one can use any tumor cell or tissue in order to establish clinical…
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TopoGEN has extensive experience with assays for topoisomerase inhibition in vivo. This analysis allows the investigator to ascertain whether a novel agent is active against endogenous Top2bin whole cells. An important benefit to this analysis is that one can use any tumor cell or tissue in order to establish clinical efficacy against a specific tumor […]
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TG2000GKIT
The E. coli DNA Gyrase and Relaxed DNA Assay Kit  is designed to provide the investigator quick and specific assays for DNA gyrase.  These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work.  These kits are also appropriate for the…
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The E. coli DNA Gyrase and Relaxed DNA Assay Kit  is designed to provide the investigator quick and specific assays for DNA gyrase.  These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work.  These kits are also appropriate for the researcher who is interested in large scale […]
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TG1040-1
TopI-NucEx Kit is designed to allow a researcher to prepare a nuclear extract enriched for topoisomerase I (top1) activity and intact 100 kDa polypeptide.  The appropriate assay buffer specific for topo I is included.
$405.00
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TG1050-1
TopII-NucEx Kit is designed to allow a researcher to prepare a nuclear extract enriched for topoisomerase II (top2) activity.  The appropriate assay buffer specific for topo II is included.
$410.00
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TG1020
High throughput In vivo Complex of Enzyme (ICE) Assay that measures endogenous topoisomerase/genomic DNA covalent complexes.  The one day procedure does not require a CsCl centrifugation step. The ICE Assay Kit is topo specific since we provide antibody that corresponds to the topo/DNA complex.
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TG4060
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).  The ability of each lot to function as an effective topoisomerase denaturant is tested and verified for each lot.  
$95.00
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TG4038
Vial contains 1ml of 10X agarose gel loading buffer ( 0.005% bromophenol blue, 50% glycerol).    
$95.00
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TG4065
20% Sarkosyl
$95.00
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TG4035
20mM ATP supplied in aqueous buffer (0.5ml).
$95.00
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