The E. coli DNA Gyrase and Relaxed DNA Assay Kit is designed to provide the investigator quick and specific assays for DNA gyrase. These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work. These kits are also appropriate for the researcher who is interested in large scale screening for novel anti-gyrase active agents. We matched the amount of DNA gyrase enzyme with the amount of Relaxed pHOT-1 DNA to get you going and keep you going on the hunt for new actives and leads.
- Topotecan Hydrochloride (Hycamtin)
- Camptothecin
- 9-Nitro-20 (S) Camptothecin (Rubitecan)
- 10-Hydroxy Camptothecin
- 9-Amino Camptothecin
- Doxorubicin (Adriacin)
- Daunomycin
- Etoposide (VP16)
- Amsacrine (m-AMSA)
- Genistein
- Ellipticine
- HU-331
Gyrase & Topo Iv
- Ciprofloxacin
- Topoisomerase II Alpha C-Terminal Peptide
- Polyclonal Antibody to Human Topoisomerase II Alpha
- Monoclonal Antibody to Human Topoisomerase II Alpha
- Polyclonal Antibody to Human Topoiosomerase II Beta
- Polyclonal Antibody to Yeast Topoisomerase II (Serum)
- Monoclonal Antibody to Human Topoiosomerase II Beta
- Topoisomerase II Immunoflourescence Kit
- Topoisomerase II Immunoprecipitation Kit
- Topoisomerase II Band Shift Kit
Cell Context DNA Repair
- Human: HeLa Reporter Cell Line (Includes I-Sce1 Transfection)
- Human: HeLa HR Reporter Cell line (Includes CRISPR-CAS Transfection)
- Human: Neuroblastoma HR Reporter Cell line (SH-SY5Y, Includes I-Sce1 Transfection)
- Mouse: 3T3 HR Reporter Cell Line (Includes I-Sce1 Transfection)
- Mouse: Neuronal Lineage HR Reporter Cell line (Includes I-Sce1 Transfection)
- Custom Cell Based Screening Kit for (HR) Pathway
- Human:HeLa NHEJ Reporter Cell Line (Includes I-Sce1 Transfection)
- Human:HNSH NHEJ Reporter Cell line (Includes I-Sce1 Transfection)
- Mouse:3T3 Fibroblast NHEJ Reporter (Includes I-Sce1 Transfection)
- Custom Cell Based Screening Kit for NHEJ
Topo Enzymes
DNA Gyrases
Topo Kits
Drug Screening Kits
Topo Antibodies
Protein Mw Marker & Antiobody Kits
DNA Substrates
TDP1 Products
TDP2 Products
Topo Related Drugs
Stable Cell Lines for DNA Repair Assays
Transfection-based DNA Repair Assays
Topo Enzymes
DNA Gyrases
Topo Kits
Drug Screening Kits
Topo Antibodies
Protein Mw Marker & Antiobody Kits
DNA Substrates
Top2/Gyrase Substrates & Markers
Top1 pHOT-1 DNA Substrates & Markers
TDP1 Products
TDP2 Products
Topo Related Drugs
Stable Cell Lines for DNA Repair Assays
These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based DNA Repair Assays
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
- It uses GFP readout to measure the efficiency of repair.
- There is zero background when repair is blocked or inhibited
- The reporters for HR or NHEJ can be installed in virtually any cell line that can be transiently transfect
- It is rapid and robust
- It is ideal for drug screens to find novel inhibitors of HR/NHEJ
- The kits are highly specific and can only report HR or NHEJ
- Readout GFP can be done by FACS or Imaging
- Entire pathway can be followed by live imaging in viable cells
- Ideal to track repair kinetics at the single cell level
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