Volumes are given for the 100 assay kit size.  For the 250 assay kit size, multiply volumes by 2.5 fold.  Note that this kit does not include Tyrosyl DNA Phosphodiesterase, which is sold separately (see link below).  We recommend reconstructing the assay using the purified Tdp1 to ensure that the assay is optimized and working well.

• Oligo-Tyr substrate (10 ul at 0.8 ug/ul in TE buffer [10 mM Tris, pH 7.5, 1 mM EDTA]).  This substrate should be end labeled with T4 polynucleotide kinase and g-[32P]- ATP as described in the kit protocol
• 5x Tdp1 assay buffer (300 ul): 5x buffer contains 100 mM Tris-HCl (pH 7.5), 500 mM KCl, 50 mM EDTA, 5mM dithiothreitol (DTT).
• 2x Stop Buffer (gel loading buffer) (200 ul): 96% formamide, 20 mM EDTA, 0.03% xylene cyanol, 0.03% bromophenol blue.