What We Do
Providing Products & Services for Cancer Research
TopoGEN is a research diagnostics company that provides innovative products and services for researchers working with topoisomerases and engaged in mechanism based drug discovery and development. Drug Screening Kits are available to identify topoisomerase active drugs for use in cancer chemotherapy and antibacterial applications. The company also provides assay kits for assaying both prokaryotic and eukaryotic topoisomerases (type I, II, IV, gyrase), both in vitro and in vivo. We offer Turn-Key contract service to screen for new Topo Drugs. As an Experienced Contract Research Organization, the advantages are numerous!
Additionally, we produce quality topoisomerases & related assay and drug screening kits as technology transfer platforms from our lab to yours. When you become our customer, we become your partner in research. We review your data and offer valuable input.
accelerate your research productivity with our
Custom Screening Services
HUMAN TOPO SCREENING IN VITRO
Do have a drug that might target human topoisomerases?
We can help! Let us design a tractable screen to validate your novel small molecule or natural product. We do this routinely for researchers who want high quality, unambiguous results in a short time frame. Go HERE to fill out a screening request and we can generate a quote.
BACTERIAL GYRASE & TOPO IV SCREENING
Do have a drug that might target bacterial DNA Gyrase or Topo IV? Also, you may need to test whether your new antibiotic drug crosses over and alters the human enzyme, Top2. Let us design a tractable screen to validate and test for antibiotic activity. Go HERE to fill out a screening request and we can generate a quote.
CELL CONTEXT TOPOISOMERASE SCREENING (ICE ASSAYS)
A key piece in drug discovery is actually showing that a new drug works in live cells. We can do this for you (in a cell line of your choosing) or you can purchase our ICE Assay Kit (Cat #TG1020) for your own lab. We have a number of different cell lines (please enquire) or you can send us your cells; either way we’re here to help.
BIO-CORRELATIONS IN CLINICIAL TRIALS
We can assist in clinical trials by measuring Topo levels or using ICE to examine Topo/DNA complexes. These are good biomarkers for clinical trials. Let us know how we can help.
WESTERN BLOTTING SERVICES
TopoGEN can quantify topoisomerase I, IIa, IIb and III levels in your extracts using Western Blotting and utilize our immune-reagents for cell distribution or localization experiments. We can also provide precise directions on making nuclear extracts and we will quantify topo levels by high resolution Western Blotting. The data will be publication quality with markers and precise quantitation.
QUANTIFY TOPOISOMERASE ACTIVITY LEVELS IN CRUDE EXTRACTS
We can quantify total topoisomerase I, II activity levels in your specific model cell line or help you prepare nuclear extracts. We will quantify topo levels using biochemical assays (relaxation, decatenation or even Westerns for total topo protein levels). We carry out titrations, quantify band intensities and analyze the data.
TOPOISOMERASE GENOMIC DNA COMPLEXES
Interested in a bioinformatic analysis of covalent Top2 or Top1/genomic DNA complexes isolated from chromatin in any given cell line? We can help. We use validated “Topo-CHIP” technologies that rely on anti-topoisomerase antibodies to immunoprecipitated the attached DNA.
Kits
Human topoisomerase assay kits and drug screening kits
Bacterial DNA Gyrase and Topo IV assay and drug screening kits
In Situ (cell context) testing of endogenous topo activity for drug validation
Western, IHC, IF and IP Kits now available
Nuclear Extraction Kits with detailed protocols on how to make Nuc. Extracts
Robust consulting and product support facilitates tech transfer (including data analysis services)
Cell Context Screens for DNA Repair (HR and NHEJ) using I-Sce enabled protocols
Replenishment support for all Kits
Technical protocols and FAQ to help you
Products
All of our products are validated & certified with Topo-enzymes
