All Products, Such as DNA substrates, are carefully validated for use with topoisomerases and gyrases. TopoGEN's enymes are high purity and consistent.
These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
We can tractably identify novel Topo poisons and catalytic inhibitors. Got a grant proposal due? We offer rapid turn around services! All data controlled, documented and certified as publication quality. Your lab may lack expertise and experience, let us help stretch your grant dollars! We can report back in 2-4 business days with a full report. We offer services for Top1, Top2A DNA Gyrase and TopIV.
We are a comprehensive source for all eukaryotic topo antibodies.
Fully validated from in vitro and in situ (Cell context use)
Screens that identify novel homology repair or non homology repair druggable targets. Modular screens can be placed inside your cell of choice. Identify new drugs or endogenous protein factors that Regulate/Target DNA repair. Relies on I-SCE1 specific DS breaks to initiate a specific repair pathway.
All Products, Such as DNA substrates, are carefully validated for use with topoisomerases and gyrases. TopoGEN's enymes are high purity and consistent.
Our Kits are optimized for ease of use. They are well controlled with clear documentation. WE OFFER ON-LINE SUPPORT!
Our Contract R&D services include cell context and in vitro HTS for rapidly finding Learn More and optimizing new topo or gyrase drugs. We save you time and money. We have years of experience!
The Company staff are true experts! Dr. Muller and his team have published extensively in the topoisomerase field and in fact invented many of the protocols. You won't be disappointed!
Senior Researcher
We were especially pleased with TopoGEN's new endogenous repair cell lines. Great system to interrogate DNA repair in a cellular context.
Technician
A great consulting group and very helpful in data analysis.
Their CRO service is worth every penny!! We all know R&D is not cheap but we got a great return on the investment.
lab director
This company performed a contract for us (screening novel compounds for DNA Gyrase activity). We learned so much from their experts. You can expect prompt results with clear interpretation. In our case, the results identified several new antibiotics leading to new IP for my.
(VP Research)
5 stars! TopoGEN Staff were so helpful in getting us going on our topo screening campaign! Thank you!
lab chief
Not many RUO companies offer such great product support. TopoGEN is unique in this regard. We got hands-on personal attention. The staff even helped us analyze and interpret our results. Kudos to all.
Senior Scientist