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Topo Antibodies

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TG2012-2
Antibody was raised to human topo I (purified from placenta) using New Zealand White rabbits.  Serum was collected and processed using standard methods.  The antibody can be used in Western blotting. Cross reactivity of this antibody with other species has not yet been tested.  It is important…
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Antibody was raised to human topo I (purified from placenta) using New Zealand White rabbits.  Serum was collected and processed using standard methods.  The antibody can be used in Western blotting. Cross reactivity of this antibody with other species has not yet been tested.  It is important to note that human topoisomerase I undergoes spontaneous […]
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TG2012-4
This monoclonal antibody was raised to human topo I (TG2005H-RC1) using standard methods.  The antibody (IgG class) works very well for Western blotting experiments.  The epitope has not been mapped.  This product has not been tested for IP or IF applications; however, given the high specificity seen…
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This monoclonal antibody was raised to human topo I (TG2005H-RC1) using standard methods.  The antibody (IgG class) works very well for Western blotting experiments.  The epitope has not been mapped.  This product has not been tested for IP or IF applications; however, given the high specificity seen on Westerns, it should work well in these […]
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TG2012
Some scleroderma patients produce high titer antibody directed against human topoisomerase I (Shero et al., 1986).  The immunoglobulin fraction was purified from these patients and shown to react specifically with the 100 kDa form of Topo I using Western blotting.  The immune complexes produced with this reagent will…
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Some scleroderma patients produce high titer antibody directed against human topoisomerase I (Shero et al., 1986).  The immunoglobulin fraction was purified from these patients and shown to react specifically with the 100 kDa form of Topo I using Western blotting.  The immune complexes produced with this reagent will bind [125I]-protein A.  When intact topoisomerase I […]
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TG2011-2
This vial contains a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase IIa.  The purity of the peptide was greater than 95% as determined by HPLC and mass spectral analysis.  Approximately 10 mg of purified peptide were coupled (through a terminal cysteine thiol) to…
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This vial contains a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase IIa.  The purity of the peptide was greater than 95% as determined by HPLC and mass spectral analysis.  Approximately 10 mg of purified peptide were coupled (through a terminal cysteine thiol) to KLH with a heterobifunctional cross-linking agent MBS […]
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TG2011-1
Rabbit Polyclonal Antibody to Human Topoisomerase II Alpha was prepared against a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase II. The purity of the peptide was greater than 95% as determined by HPLC and mass spectral analysis. The antibody titer was determined by ELISA to…
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Rabbit Polyclonal Antibody to Human Topoisomerase II Alpha was prepared against a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase II. The purity of the peptide was greater than 95% as determined by HPLC and mass spectral analysis. The antibody titer was determined by ELISA to be > 1:104. Immunoaffinity gel […]
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TG2010-1
Mouse monoclonal antibody prepared against an oligopeptide derived from the C-terminus of the p170 kDa human Topoisomerase II Alpha.  The antibody was then affinity purified over a column containing the same peptide.    
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TG2010-3
There are at least two type II topoisomerases in animal cells: Topo IIa and Topo IIb (Chung et al., 1989; Tan et al., 1992).  The former is a cell cycle regulated enzyme of 170 kDa and the latter is not related to cell cycle phase and is slightly larger…
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There are at least two type II topoisomerases in animal cells: Topo IIa and Topo IIb (Chung et al., 1989; Tan et al., 1992).  The former is a cell cycle regulated enzyme of 170 kDa and the latter is not related to cell cycle phase and is slightly larger (180 kDa).  This rabbit polyclonal antibody […]
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TG2014
Topoisomerase II was purified from Saccharomyces cerevisiae.  A homogeneous preparation of the enzyme was injected into New Zealand white rabbits and the animals were boosted twice more.  ELISA titers were greater than 1:10 5 at the time of the final bleed.  The sera were collected and tested…
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Topoisomerase II was purified from Saccharomyces cerevisiae.  A homogeneous preparation of the enzyme was injected into New Zealand white rabbits and the animals were boosted twice more.  ELISA titers were greater than 1:10 5 at the time of the final bleed.  The sera were collected and tested by probing Western blots. A single polypeptide band […]
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TG2011-5
Mouse Monoclonal Antibody to Human Topoisomerase II Beta: Western Blotting and Immunohistochemistry Reagent There are at least two type II topoisomerases in animal cells: Topo IIaand Topo IIb;  The former is a cell cycle regulated enzyme of 170 kDa and the latter is not related to cell cycle phase and…
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Mouse Monoclonal Antibody to Human Topoisomerase II Beta: Western Blotting and Immunohistochemistry Reagent There are at least two type II topoisomerases in animal cells: Topo IIaand Topo IIb;  The former is a cell cycle regulated enzyme of 170 kDa and the latter is not related to cell cycle phase and is slightly larger (180 kDa). […]
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TG2010-4
A rabbit polyclonal antibody was prepared against a pool of oligopeptides derived from the carboxy terminal region of human topoisomerase IIIb.  The purity of the peptides was greater than 95% as determined by HPLC and mass spectral analysis.  Approximately 10 mg of purified peptide were coupled (through a…
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A rabbit polyclonal antibody was prepared against a pool of oligopeptides derived from the carboxy terminal region of human topoisomerase IIIb.  The purity of the peptides was greater than 95% as determined by HPLC and mass spectral analysis.  Approximately 10 mg of purified peptide were coupled (through a terminal cysteine thiol) to KLH with a […]
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TG2015
This monoclonal antibody was raised against purified recombinant protein and is specific for the C-terminal region (residues 748-976).  The antibody is not ideal for use in western blotting experiments but will work on slot blot applications.  It is well suited for immunohistochemistry with paraffin embedded tissue using 1:25…
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This monoclonal antibody was raised against purified recombinant protein and is specific for the C-terminal region (residues 748-976).  The antibody is not ideal for use in western blotting experiments but will work on slot blot applications.  It is well suited for immunohistochemistry with paraffin embedded tissue using 1:25 to 150 dilutions, the high temperature unmasking […]
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TG1030
This assay kit contains all the reagents for cytolocalization of Human Top2a using immunofluorescence.  The system is optimized for use with FITC or Rhodamine labeled secondary antibody with adherent cells.  The method may work with other cells besides human. Mouse, rat and monkey cells will cross react with…
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This assay kit contains all the reagents for cytolocalization of Human Top2a using immunofluorescence.  The system is optimized for use with FITC or Rhodamine labeled secondary antibody with adherent cells.  The method may work with other cells besides human. Mouse, rat and monkey cells will cross react with topo II antibody.  The antibody is specific […]
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TG1035
The immunoprecpitation (IP) kit contains the key reagents and protocol for immunoprecipitating Human Top2a.  The method should work well with other cells besides humans.  For example, mouse, rat and monkey cells will cross react with topoisomerase II antibody.  The antibody is specific for p170 (topo II alpha)…
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The immunoprecpitation (IP) kit contains the key reagents and protocol for immunoprecipitating Human Top2a.  The method should work well with other cells besides humans.  For example, mouse, rat and monkey cells will cross react with topoisomerase II antibody.  The antibody is specific for p170 (topo II alpha) in human (HeLa) cells and although we presume […]
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TG1024
TopoGEN has extensive experience with assays for topoisomerase inhibition in vivo. This analysis allows the investigator to ascertain whether a novel agent is active against endogenous Top2bin whole cells. An important benefit to this analysis is that one can use any tumor cell or tissue in order to establish clinical…
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TopoGEN has extensive experience with assays for topoisomerase inhibition in vivo. This analysis allows the investigator to ascertain whether a novel agent is active against endogenous Top2bin whole cells. An important benefit to this analysis is that one can use any tumor cell or tissue in order to establish clinical efficacy against a specific tumor […]
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TG2011-3
The marker set for topo II is used to unambiguously locate the position of the 170 kDa topo II in Western Blots. The human 170 kDa is present in SDS-PAGE loading buffer.  Note that human topo II may break down spontaneously with prolonged storage or with repeated freezing and…
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The marker set for topo II is used to unambiguously locate the position of the 170 kDa topo II in Western Blots. The human 170 kDa is present in SDS-PAGE loading buffer.  Note that human topo II may break down spontaneously with prolonged storage or with repeated freezing and thawing cycles.  TopoGEN, Inc. stipulates that […]
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