TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages). Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.
SKU: TG4130 Categories: Topo II, Topoisomerase Related Drugs Tag: daunomycin
Chemical Name: (8S-cis)-8-Acetyl-10-[(3-amino-2,3,6-trideoxy-α-L- lyxohexopyranosyl) oxy]-7,8,9,10-tetrahydro-6,8,11- trihydroxy-1-methoxy-5,12-naphthacenedione
Molecular Formula: C27H29NO10 · HCl
Molecular Weight: 563.98
TG4130 is supplied already suspended in water at 10 mM (0.25 ml) and is ready for use. The drug is pre-tested and shown to induce topo cleavages using purified human topoisomerase from TopoGEN.
The liquid product is shipped on blue ice. Store at -20°C.
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