Irinotecan (also known as CPT-11) is a water soluble, semi-synthetic analogue of 20(S)camptothecin (CPT) with promising activity against a range of tumour types. Irinotecan causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. [Clin Exp Pharmacol Physiol. 1996 Oct-Nov;23(10-11):1000-4.] TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages). Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.
SKU: TG4101 Category: Topoisomerase Related Drugs Tags: cpt11, irinotecan
Molecular Formula: C33H38N4O6.HCl.3H2O
Molecular Weight: 677.2
TG4101 is supplied already suspended in water at 10 mM (0.25 ml) and is ready for use. The drug is pre-tested and shown to induce topo cleavages using purified human topoisomerase from TopoGEN.
The liquid product is shipped on dry ice. Store at -20°C.
If you require additional information about this product, please contact us at: email@example.com
Additional Information & Media relating to Product