This vial contains a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase IIa. The purity of the peptide was greater than 95% as determined by HPLC and mass spectral analysis. Approximately 10 mg of purified peptide were coupled (through a terminal cysteine thiol) to KLH with a heterobifunctional cross-linking agent MBS at a ratio of 1 part peptide to 1 part KLH. The peptide was used to immunize rabbits for antibody production. The resulting antibody reacts strongly and specifically with the 170 kDa Human Topo II protein. This peptide is used as a control reagent in immunofluorescence; it effectively neutralizes the antibody to topo II.
- Topotecan Hydrochloride (Hycamtin)
- Camptothecin
- 9-Nitro-20 (S) Camptothecin (Rubitecan)
- 10-Hydroxy Camptothecin
- 9-Amino Camptothecin
- Doxorubicin (Adriacin)
- Daunomycin
- Etoposide (VP16)
- Amsacrine (m-AMSA)
- Genistein
- Ellipticine
- HU-331
Gyrase & Topo Iv
- Ciprofloxacin
- Topoisomerase II Alpha C-Terminal Peptide
- Polyclonal Antibody to Human Topoisomerase II Alpha
- Monoclonal Antibody to Human Topoisomerase II Alpha
- Polyclonal Antibody to Human Topoiosomerase II Beta
- Polyclonal Antibody to Yeast Topoisomerase II (Serum)
- Monoclonal Antibody to Human Topoiosomerase II Beta
- Topoisomerase II Immunoflourescence Kit
- Topoisomerase II Immunoprecipitation Kit
- Topoisomerase II Band Shift Kit
Cell Context DNA Repair
- Human: HeLa Reporter Cell Line (Includes I-Sce1 Transfection)
- Human: HeLa HR Reporter Cell line (Includes CRISPR-CAS Transfection)
- Human: Neuroblastoma HR Reporter Cell line (SH-SY5Y, Includes I-Sce1 Transfection)
- Mouse: 3T3 HR Reporter Cell Line (Includes I-Sce1 Transfection)
- Mouse: Neuronal Lineage HR Reporter Cell line (Includes I-Sce1 Transfection)
- Custom Cell Based Screening Kit for (HR) Pathway
- Human:HeLa NHEJ Reporter Cell Line (Includes I-Sce1 Transfection)
- Human:HNSH NHEJ Reporter Cell line (Includes I-Sce1 Transfection)
- Mouse:3T3 Fibroblast NHEJ Reporter (Includes I-Sce1 Transfection)
- Custom Cell Based Screening Kit for NHEJ
Topo Enzymes
DNA Gyrases
Topo Kits
Drug Screening Kits
Topo Antibodies
Protein Mw Marker & Antiobody Kits
DNA Substrates
TDP1 Products
TDP2 Products
Topo Related Drugs
Stable Cell Lines for DNA Repair Assays
Transfection-based DNA Repair Assays
Topo Enzymes
DNA Gyrases
Topo Kits
Drug Screening Kits
Topo Antibodies
Protein Mw Marker & Antiobody Kits
DNA Substrates
Top2/Gyrase Substrates & Markers
Top1 pHOT-1 DNA Substrates & Markers
TDP1 Products
TDP2 Products
Topo Related Drugs
Stable Cell Lines for DNA Repair Assays
These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based DNA Repair Assays
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
- It uses GFP readout to measure the efficiency of repair.
- There is zero background when repair is blocked or inhibited
- The reporters for HR or NHEJ can be installed in virtually any cell line that can be transiently transfect
- It is rapid and robust
- It is ideal for drug screens to find novel inhibitors of HR/NHEJ
- The kits are highly specific and can only report HR or NHEJ
- Readout GFP can be done by FACS or Imaging
- Entire pathway can be followed by live imaging in viable cells
- Ideal to track repair kinetics at the single cell level
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