These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
Here at TopoGEN we foster a ongoing learning environment to train students (from high school to university level) who wish to learn hands-on the technical and intellectual aspects of biotech. We welcome applications from highly motivated students interested in a future career with the Company or help with making career decisions in our field. In addition to hands-on training, interns and trainees will learn the science behind their project. Contact us if you are interested in either working as an intern for the summer or academic year or in a more lengthy relationship of employment. You won’t be disappointed!!. For those seeking a long term employment track with TopoGEN, we encourage you to apply. We pride ourselves on our ability to teach and train incoming technical staff (the Company was founded by former academics (university faculty, specifically) and we are seasoned educators). Life in the company is characterized by ongoing learning (seminars, paper presentations) and laboratory work. We offer high flexible hours and make efforts to create independent researchers and critical thinking about science. Let us know if you have an interest in a career path with TopoGEN.
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