TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages). Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.
SKU: TG4120 Categories: Topo II, Topoisomerase Related Drugs Tags: adriacin, dox, doxorubicin
Molecular Name: (8S-cis)10-[(3-Amino-2,3,6-trideoxy-α-L-lyxo- hexopyranosyl)oxy-7,8,9,10-tetrahydro-6,8,11- trihydroxy-8-(hydroacetyl)-1-methoxy-5,12- naphthacenedione
Molecular Formula: C27H29NO11 .HCl
Molecular Weight: 579.99
TG4120 is supplied already suspended in water at 10 mM (0.25 ml) and is ready for use. The drug is pre-tested and shown to induce topo cleavages using purified human topoisomerase from TopoGEN.
The liquid product is shipped on blue ice. Store at -20°C.
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