The TopII-NucEx Kit is designed to allow a researcher to prepare a nuclear extract enriched for type II topoisomerase (topo II) activity. The appropriate assay buffer specific for topo II is included. This kit is suitable for most animal cell lines growing in vitro but is optimized for human and rodent species. This Kit also allows the investigator to quantify topo II activity in any given cell line. TopII-NucEx Kit provides the end user a source of enzyme for preliminary drug screening for topoisomerase II active agents.
In summary, this kit contains everything needed to assay topo II in a crude extract (including kDNA, buffers and markers) and everything needed to make the crude nuclear extract from adherent cell lines (HeLa). The extraction method can be adopted to tissues or other cell lines with some optimization. The kit is suitable for drug screening experiments as noted. We recommend that drug controls (+/- Etoposide) be included for all drug screening experiments. Topo II drugs are available on our website.