Topoisomerase II Assay Buffer


Topo II Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use:

  • 10X Incomplete Topo II Assay Buffer A contains the following: 0.5M Tris-HCl (pH8.0), 1.5M NaCl, 0.1M MgCl2, 5 mM dithiothreitol.
  • 10X ATP Buffer B contains 20 mM ATP in water.

These two buffers must be mixed to prepare a final 5X working stock of assay buffer.  Furthermore, the final 5X Complete Buffer contains both A and B and should be made fresh for each experiment.  Do not freeze the 5X Complete Buffer; it is stable for 24 hours on ice.  To Prepare the 5X Complete Buffer, mix equal volumes of A and B (0.1ml of A and 0.1ml of B) and place at 4° C or on ice.

Quality Control Analysis:
The 5X Complete Topo II Assay Buffer (containing equal parts A and B above) is optimized and tested for decatenation and relaxation activity of eukaryotic topo II.  The Complete Buffer should be made fresh for each

The two-part buffer is shipped at room temperature (or on dry ice if included with enzyme) but should be stored at -20°C. The 5X Topo II Complete Assay Buffer is stable for 24 hours at 4°C.

Topoisomerase II Assay Buffer Data Sheet

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