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Product ID | Product Info | Description |
|---|---|---|
TG1017 |
This kit is designed to allow the customer to screen for agents (drugs, natural products, small molecules, synthetics) that block the catalytic activity of human topoisomerase I (topo I). The use of high stoichiometric ratios of topo I:DNA induces cleavage complexes without the need to use topo I poisons… Show more (+) This kit is designed to allow the customer to screen for agents (drugs, natural products, small molecules, synthetics) that block the catalytic activity of human topoisomerase I (topo I). The use of high stoichiometric ratios of topo I:DNA induces cleavage complexes without the need to use topo I poisons (camptothecin) which facilitates detection of catalytic […] Show less (-) |
|
TG1019 |
This kDNA based Topo II Drug Screening Kit is designed to identify agents that target type II topoisomerases acting as catalytic inhibitors, primarily; however, poisons can also be detected with this kit. |
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TG1018 |
The Topo I Drug Kit is designed to allow the investigator to quickly identify novel inhibitors of Human Top1. The kit will allow detection of novel compounds that either stabilize the nicked intermediate or otherwise inhibit catalytic activity of topoisomerase I. |
|
TG1009 |
This is a cleavage based detection kit that will reveal whether a particular agent either antagonizes the action of topo II or affects the cleavage/religation cycle. Reactions are assembled in microcentrifuge tubes using reagents supplied in the kit (buffer, DNA, control inhibitor). |
|
TG1007 |
The Topoisomerase IV Drug Screening Kit contains reagents necessary to identify compounds that affect enzyme activity. Note that kits with a catalog number denoted -A will include E. coli topoisomerase IV enyzme. |
|
TG4039 |
Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at… Show more (+) Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at elevated temperatures up to 65° C. Calcium […] Show less (-) |
|
TG4037 |
Is used to terminate topoisomerase reactions. To terminate reactions, add 1/5 volume to the reaction prior to loading agarose gel. |
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TG4090 |
Topo II Dilution Buffer is supplied as a working solution at 1X solution. The buffer is optimized and tested for use as a diluent for TopoGEN’s type II topoisomerase. |
|
TG4060 |
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v). The ability of each lot to function as an effective topoisomerase denaturant is tested and verified for each lot. |
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TG4045 |
S. Aureus Gyrase Assay Buffer (1ml). |
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TG4032 |
Topo IV Assay Buffer is supplied as a 5x Stock solution in two parts that must be mixed prior to use: These two buffers must be mixed to prepare a final 5x complete Topo IV Assay Buffer (A+B) as follows: add 0.1 volume of Buffer B to 1.0… Show more (+) Topo IV Assay Buffer is supplied as a 5x Stock solution in two parts that must be mixed prior to use: These two buffers must be mixed to prepare a final 5x complete Topo IV Assay Buffer (A+B) as follows: add 0.1 volume of Buffer B to 1.0 volume Buffer A. Prepare only as much […] Show less (-) |
|
TG4038 |
Vial contains 1ml of 10X agarose gel loading buffer ( 0.005% bromophenol blue, 50% glycerol). |
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TG4040 |
Topo II Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use: 10X Incomplete Topo II Assay Buffer A contains the following: 0.5M Tris-HCl (pH8.0), 1.5M NaCl, 0.1M MgCl2, 5 mM dithiothreitol. 10X ATP Buffer B contains 20… Show more (+) Topo II Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use: 10X Incomplete Topo II Assay Buffer A contains the following: 0.5M Tris-HCl (pH8.0), 1.5M NaCl, 0.1M MgCl2, 5 mM dithiothreitol. 10X ATP Buffer B contains 20 mM ATP in water. These two buffers must be […] Show less (-) |
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TG4055 |
5X Potassium Glutamate (1ml). |
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TG4065 |
20% Sarkosyl |
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TG4095 |
Topo I Dilution Buffer is supplied as a working solution at 1X solution. The buffer is optimized and tested for use as a diluent for recombinant human topoisomerase I enzyme from TopoGEN. |
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TG4030 |
E. coli DNA gyrase assay buffer supplied as a 5X stock solution. The buffer is optimized and tested for E. coli DNA gyrase only. |
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TG4035 |
20mM ATP supplied in aqueous buffer (0.5ml). |
|
TG1004 |
The Tdp1 Assay Kit contains all reagents for routine detection of Tdp1 activity using the tyrosine release assay from the substrate Oligo-Tyr (human tdp1 is sold separately). The assay is highly specific for Tdp1. Summary: The kit contains reagents and protocols for assaying Tyrosyl DNA Phosphodiesterase (Tdp1). A procedure… Show more (+) The Tdp1 Assay Kit contains all reagents for routine detection of Tdp1 activity using the tyrosine release assay from the substrate Oligo-Tyr (human tdp1 is sold separately). The assay is highly specific for Tdp1. Summary: The kit contains reagents and protocols for assaying Tyrosyl DNA Phosphodiesterase (Tdp1). A procedure for making suitable cell extracts is described […] Show less (-) |
|
TG2004H |
TopoGEN provides purified Human Tyrosyl DNA Phosphodiesterase (Tdp1) purified to homogeneity to a single 68 kDa band on SDS-PAGE. Tdp1 is catalytically active and will remove a terminal 3’ phosphotyrosyl group from a specifically designed oligo target site (oligo-Tyr). Additionally, TopoGEN scientists have demonstrated that Tdp1 will release… Show more (+) TopoGEN provides purified Human Tyrosyl DNA Phosphodiesterase (Tdp1) purified to homogeneity to a single 68 kDa band on SDS-PAGE. Tdp1 is catalytically active and will remove a terminal 3’ phosphotyrosyl group from a specifically designed oligo target site (oligo-Tyr). Additionally, TopoGEN scientists have demonstrated that Tdp1 will release topo I covalently attached to genomic DNA […] Show less (-) |
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