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TG1019
This kDNA based Topo II Drug Screening Kit is designed to identify agents that target type II topoisomerases acting as catalytic inhibitors, primarily; however, poisons can also be detected with this kit.
TG1018
The Topo I Drug Kit is designed to allow the investigator to quickly identify novel inhibitors of Human Top1.  The kit will allow detection of novel compounds that either stabilize the nicked intermediate or otherwise inhibit catalytic activity of topoisomerase I.  
TG1009
This is a cleavage based detection kit that will reveal whether a particular agent either antagonizes the action of topo II or affects the cleavage/religation cycle.  Reactions are assembled in microcentrifuge tubes using reagents supplied in the kit (buffer, DNA, control inhibitor).
TG1007
The Topoisomerase IV Drug Screening Kit contains reagents necessary to identify compounds that affect enzyme activity.  Note that kits with a catalog number denoted -A will include E. coli topoisomerase IV enyzme.    
TG2001GKIT
This kit contains purified bacterial (E. coli) DNA Gyrase  purified to homogeneity (>98% based on SDS-PAGE). The Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex.  The enzyme is supplied at the unit concentration given on the quality control data provided…
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This kit contains purified bacterial (E. coli) DNA Gyrase  purified to homogeneity (>98% based on SDS-PAGE). The Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex.  The enzyme is supplied at the unit concentration given on the quality control data provided with the kit.  It is also stored […]
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TG1017
This kit is designed to allow the customer to screen for agents (drugs, natural products, small molecules, synthetics) that block the catalytic activity of human topoisomerase I (topo I).  The use of high stoichiometric ratios of topo I:DNA induces cleavage complexes without the need to use topo I poisons…
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This kit is designed to allow the customer to screen for agents (drugs, natural products, small molecules, synthetics) that block the catalytic activity of human topoisomerase I (topo I).  The use of high stoichiometric ratios of topo I:DNA induces cleavage complexes without the need to use topo I poisons (camptothecin) which facilitates detection of catalytic […]
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TG4032
Topo IV Assay Buffer is supplied as a 5x Stock solution in two parts that must be mixed prior to use: These two buffers must be mixed to prepare a final 5x complete Topo IV Assay Buffer (A+B) as follows: add 0.1 volume of Buffer B to 1.0…
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Topo IV Assay Buffer is supplied as a 5x Stock solution in two parts that must be mixed prior to use: These two buffers must be mixed to prepare a final 5x complete Topo IV Assay Buffer (A+B) as follows: add 0.1 volume of Buffer B to 1.0 volume Buffer A. Prepare only as much […]
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TG4038
Vial contains 1ml of 10X agarose gel loading buffer ( 0.005% bromophenol blue, 50% glycerol).    
TG4040
Topo II Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use: 10X Incomplete Topo II Assay Buffer A contains the following: 0.5M Tris-HCl (pH8.0), 1.5M NaCl, 0.1M MgCl2, 5 mM dithiothreitol. 10X ATP Buffer B contains 20…
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Topo II Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use: 10X Incomplete Topo II Assay Buffer A contains the following: 0.5M Tris-HCl (pH8.0), 1.5M NaCl, 0.1M MgCl2, 5 mM dithiothreitol. 10X ATP Buffer B contains 20 mM ATP in water. These two buffers must be […]
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TG4055
5X Potassium Glutamate (1ml).
TG4065
20% Sarkosyl
TG4095
Topo I Dilution Buffer is supplied as a working solution at 1X solution.  The buffer is optimized and tested for use as a diluent for recombinant human topoisomerase I enzyme from TopoGEN.
TG4030
E. coli DNA gyrase assay buffer supplied as a 5X stock solution.  The buffer is optimized and tested for E. coli DNA gyrase only.      
TG4035
20mM ATP supplied in aqueous buffer (0.5ml).
TG4039
Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times.  The activity of proteinase K is increased at…
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Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times.  The activity of proteinase K is increased at elevated temperatures up to 65° C. Calcium […]
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TG4037
Is used to terminate topoisomerase reactions.  To terminate reactions, add 1/5 volume to the reaction prior to loading agarose gel.      
TG4090
Topo II Dilution Buffer is supplied as a working solution at 1X solution.  The buffer is optimized and tested for use as a diluent for TopoGEN’s type II topoisomerase.        
TG4060
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).  The ability of each lot to function as an effective topoisomerase denaturant is tested and verified for each lot.  
TG4045
S. Aureus Gyrase Assay Buffer (1ml).
TG1004
The Tdp1 Assay Kit contains all reagents for routine detection of Tdp1 activity using the tyrosine release assay from the substrate Oligo-Tyr (human tdp1 is sold separately).  The assay is highly specific for Tdp1. Summary: The kit contains reagents and protocols for assaying Tyrosyl DNA Phosphodiesterase (Tdp1).  A procedure…
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The Tdp1 Assay Kit contains all reagents for routine detection of Tdp1 activity using the tyrosine release assay from the substrate Oligo-Tyr (human tdp1 is sold separately).  The assay is highly specific for Tdp1. Summary: The kit contains reagents and protocols for assaying Tyrosyl DNA Phosphodiesterase (Tdp1).  A procedure for making suitable cell extracts is described […]
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