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Topo & Drug Screening Kits
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PRODUCT ID | Product Info | Description | Purchase |
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TG4060 |
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v). The ability of each lot to function as an effective topoisomerase denaturant is tested and verified for each lot. |
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TG4038 |
Vial contains 1ml of 10X agarose gel loading buffer ( 0.005% bromophenol blue, 50% glycerol). |
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TG4065 |
20% Sarkosyl |
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TG4035 |
20mM ATP supplied in aqueous buffer (0.5ml). |
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TG4037 |
Is used to terminate topoisomerase reactions. To terminate reactions, add 1/5 volume to the reaction prior to loading agarose gel. |
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TG1025 |
The purpose of the DNA Intercalator/Unwinding Kit is to determine if a compound induces DNA unwinding characteristic of DNA intercalators. Sufficient materials are provided for at least 50 independent determinations. NOTE: enzyme is not included with this kit. |
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TG2000GKIT |
The E. coli DNA Gyrase and Relaxed DNA Assay Kit is designed to provide the investigator quick and specific assays for DNA gyrase. These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work. These kits are also appropriate for the… Show more (+) The E. coli DNA Gyrase and Relaxed DNA Assay Kit is designed to provide the investigator quick and specific assays for DNA gyrase. These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work. These kits are also appropriate for the researcher who is interested in large scale […] Show less (-) |
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TG4030 |
E. coli DNA gyrase assay buffer supplied as a 5X stock solution. The buffer is optimized and tested for E. coli DNA gyrase only. |
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TG2001GKIT |
This kit contains purified bacterial (E. coli) DNA Gyrase purified to homogeneity (>98% based on SDS-PAGE). The Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex. The enzyme is supplied at the unit concentration given on the quality control data provided… Show more (+) This kit contains purified bacterial (E. coli) DNA Gyrase purified to homogeneity (>98% based on SDS-PAGE). The Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex. The enzyme is supplied at the unit concentration given on the quality control data provided with the kit. It is also stored […] Show less (-) |
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TG1007 |
The Topoisomerase IV Drug Screening Kit contains reagents necessary to identify compounds that affect enzyme activity. Note that kits with a catalog number denoted -A will include E. coli topoisomerase IV enyzme. This kit will allow detection of two kinds of topoisomerase inhibitors: those that stimulate formation of… Show more (+) The Topoisomerase IV Drug Screening Kit contains reagents necessary to identify compounds that affect enzyme activity. Note that kits with a catalog number denoted -A will include E. coli topoisomerase IV enyzme. This kit will allow detection of two kinds of topoisomerase inhibitors: those that stimulate formation of cleavable complexes and those that antagonize topoisomerase […] Show less (-) |
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TG1003 |
The Kit is designed to allow quick and specific detection of DNA gyrase. This kit facilitates the purification and characterization of type II topoisomerase enzymes (DNA Gyrase) and contains all reagents necessary for routine assays of type II enzymes that either have or do not have the ability to supercoil… Show more (+) The Kit is designed to allow quick and specific detection of DNA gyrase. This kit facilitates the purification and characterization of type II topoisomerase enzymes (DNA Gyrase) and contains all reagents necessary for routine assays of type II enzymes that either have or do not have the ability to supercoil. Show less (-) |
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TG1015 |
This kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in… Show more (+) This kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking […] Show less (-) |
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TG1020 |
TopoGEN has extensive experience with cell-based screens for topoisomerase targeting. Our staff scientists developed and published a method for detecting covalent topo/genomic DNA complexes in live cells (1,2) that is widely utilized in the field. The method, called the ICE Assay (In vivo Complex of Enzyme) originally… Show more (+) TopoGEN has extensive experience with cell-based screens for topoisomerase targeting. Our staff scientists developed and published a method for detecting covalent topo/genomic DNA complexes in live cells (1,2) that is widely utilized in the field. The method, called the ICE Assay (In vivo Complex of Enzyme) originally required a CsCl gradient to purify genomic DNA […] Show less (-) |
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TG1001 |
This kit is designed to allow quick and specific assays for eukaryotic type II DNA topoisomerases. This kit facilitates the purification and characterization of type II enzymes and contains all reagents necessary for routine assays of type II topoisomerases. The assay is based upon decatentation of kinetoplast DNA (kDNA) and… Show more (+) This kit is designed to allow quick and specific assays for eukaryotic type II DNA topoisomerases. This kit facilitates the purification and characterization of type II enzymes and contains all reagents necessary for routine assays of type II topoisomerases. The assay is based upon decatentation of kinetoplast DNA (kDNA) and because it is specific for […] Show less (-) |
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TG4055 |
5X Potassium Glutamate (1ml). |
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TG4039 |
Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at… Show more (+) Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at elevated temperatures up to 65° C. Calcium […] Show less (-) |
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TG4045 |
S. Aureus Gyrase Assay Buffer (1ml). |
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TG4030-1 |
S. Aureus Gyrase Assay Buffer (1ml). |
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TG1040-1 |
This Kit is designed to allow a researcher to prepare a nuclear extract enriched for type I topoisomerase (topo I) activity and intact 100 kDa polypeptide. The appropriate assay buffer specific for topo I is included. This kit is suitable for most animal cell lines growing in vitro… Show more (+) This Kit is designed to allow a researcher to prepare a nuclear extract enriched for type I topoisomerase (topo I) activity and intact 100 kDa polypeptide. The appropriate assay buffer specific for topo I is included. This kit is suitable for most animal cell lines growing in vitro but is optimized for human and rodent […] Show less (-) |
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TG1050-1 |
This Kit is designed to allow a researcher to prepare a nuclear extract enriched for type II topoisomerase (topo II) activity. The appropriate assay buffer specific for topo II is included. This kit is suitable for most animal cell lines growing in vitro but is optimized for human… Show more (+) This Kit is designed to allow a researcher to prepare a nuclear extract enriched for type II topoisomerase (topo II) activity. The appropriate assay buffer specific for topo II is included. This kit is suitable for most animal cell lines growing in vitro but is optimized for human and rodent species. This Kit also allows […] Show less (-) |
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