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Topo & Drug Screening Kits

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TG4060
High purity SDS is dissolved in triple glass distilled/deionized, sterile water to 10% (w/v).  The ability of each lot to function as an effective topoisomerase denaturant is tested and verified for each lot.  
$95.00
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TG4038
Vial contains 1ml of 10X agarose gel loading buffer ( 0.005% bromophenol blue, 50% glycerol).    
$95.00
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TG4065
20% Sarkosyl
$95.00
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TG4035
20mM ATP supplied in aqueous buffer (0.5ml).
$95.00
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TG4037
Is used to terminate topoisomerase reactions.  To terminate reactions, add 1/5 volume to the reaction prior to loading agarose gel.      
$95.00
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TG1025
The purpose of the DNA Intercalator/Unwinding Kit is to determine if a compound induces DNA unwinding characteristic of DNA intercalators. Sufficient materials are provided for at least 50 independent determinations.  NOTE: enzyme is not included with this kit.  
$465.00
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TG2000GKIT
The E. coli DNA Gyrase and Relaxed DNA Assay Kit  is designed to provide the investigator quick and specific assays for DNA gyrase.  These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work.  These kits are also appropriate for the…
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The E. coli DNA Gyrase and Relaxed DNA Assay Kit  is designed to provide the investigator quick and specific assays for DNA gyrase.  These kits facilitate the purification and characterization of gyrase by providing controlled reagents that we certify to work.  These kits are also appropriate for the researcher who is interested in large scale […]
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TG4030
E. coli DNA gyrase assay buffer supplied as a 5X stock solution.  The buffer is optimized and tested for E. coli DNA gyrase only.      
$95.00
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TG2001GKIT
This kit contains purified bacterial (E. coli) DNA Gyrase  purified to homogeneity (>98% based on SDS-PAGE). The Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex.  The enzyme is supplied at the unit concentration given on the quality control data provided…
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This kit contains purified bacterial (E. coli) DNA Gyrase  purified to homogeneity (>98% based on SDS-PAGE). The Gyrase is prepared from overexpressing strains and is supplied as purified holoenzyme in an A2B2 complex.  The enzyme is supplied at the unit concentration given on the quality control data provided with the kit.  It is also stored […]
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TG1007
The Topoisomerase IV Drug Screening Kit contains reagents necessary to identify compounds that affect enzyme activity.  Note that kits with a catalog number denoted -A will include E. coli topoisomerase IV enyzme.    
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TG1003
The Kit is designed to allow quick and specific detection of DNA gyrase. This kit facilitates the purification and characterization of type II topoisomerase enzymes (DNA Gyrase) and contains all reagents necessary for routine assays of type II enzymes that either have or do not have the ability to supercoil…
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The Kit is designed to allow quick and specific detection of DNA gyrase. This kit facilitates the purification and characterization of type II topoisomerase enzymes (DNA Gyrase) and contains all reagents necessary for routine assays of type II enzymes that either have or do not have the ability to supercoil.      
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$295.00
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TG1015
The Human Topoisomerase I Assay kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction…
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The Human Topoisomerase I Assay kit contains reagents for routine detection of Top1 activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order […]
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TG1020
High throughput In vivo Complex of Enzyme (ICE) Assay that measures endogenous topoisomerase/genomic DNA covalent complexes.  The one day procedure does not require a CsCl centrifugation step. The ICE Assay Kit is topo specific since we provide antibody that corresponds to the topo/DNA complex.
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TG1001
This kit is designed to allow quick and specific assays for any type II topoisomerase, either eu- or prokaryotic. This kit facilitates the purification and characterization of type II enzymes and contains all reagents necessary for routine assays of Topoisomerase II.
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TG4055
5X Potassium Glutamate (1ml).
$95.00
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TG4039
Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times.  The activity of proteinase K is increased at…
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Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album.  It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times.  The activity of proteinase K is increased at elevated temperatures up to 65° C. Calcium […]
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$95.00
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TG4045
S. Aureus Gyrase Assay Buffer (1ml).
$95.00
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TG1040-1
TopI-NucEx Kit is designed to allow a researcher to prepare a nuclear extract enriched for topoisomerase I (top1) activity and intact 100 kDa polypeptide.  The appropriate assay buffer specific for topo I is included.
$405.00
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TG1050-1
TopII-NucEx Kit is designed to allow a researcher to prepare a nuclear extract enriched for topoisomerase II (top2) activity.  The appropriate assay buffer specific for topo II is included.
$410.00
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TG4095
Topo I Dilution Buffer is supplied as a working solution at 1X solution.  The buffer is optimized and tested for use as a diluent for recombinant human topoisomerase I enzyme from TopoGEN.
$95.00
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