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Topo Related Drugs & Assay Reagents
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PRODUCT ID | Product Info | Description | Purchase |
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TG4103 |
10-Hydroxy Camptothecin has shown promising activity against a range of tumour types. This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. TopoGEN provides this reagent as a control poison/inhibitor of… Show more (+) 10-Hydroxy Camptothecin has shown promising activity against a range of tumour types. This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I. For in vitro […] Show less (-) |
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TG4104 |
9-Amino-20-(R,S)-camptothecin (also known as 9-amino campthecin) is an analogue of camptothecin. Like other CPT congeners, it shows some promising activity against a range of tumor types. This compound causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA… Show more (+) 9-Amino-20-(R,S)-camptothecin (also known as 9-amino campthecin) is an analogue of camptothecin. Like other CPT congeners, it shows some promising activity against a range of tumor types. This compound causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. TopoGEN […] Show less (-) |
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TG4102 |
20(S)camptothecin has shown promising activity against a range of tumour types. This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. TopoGEN provides this reagent as a control poison/inhibitor… Show more (+) 20(S)camptothecin has shown promising activity against a range of tumour types. This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I. For in vitro cleavage […] Show less (-) |
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TG4150 |
TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of… Show more (+) TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive […] Show less (-) |
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TG4110 |
Camptothecin is a topo I poison that both inhibits enzyme activity and stabilizes formation of topo I cleavage complexes (Hsiang et al J. Biol. Chem. 260:14873-14878, 1985). TopoGEN provides this reagent as a control inhibitor/poison of topoisomerase I. For in vitro cleavage detection, we recommend that the drug… Show more (+) Camptothecin is a topo I poison that both inhibits enzyme activity and stabilizes formation of topo I cleavage complexes (Hsiang et al J. Biol. Chem. 260:14873-14878, 1985). TopoGEN provides this reagent as a control inhibitor/poison of topoisomerase I. For in vitro cleavage detection, we recommend that the drug be tested in the range 100 uM; […] Show less (-) |
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TG4180 |
A control poison/inhibitor of DNA Gyrase and Topo IV. This is also included in the Topo IV/Gyrase Drug Screening Kits. |
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TG4130 |
TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of… Show more (+) TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive […] Show less (-) |
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TG2020-1 |
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can… Show more (+) Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can be avoided by using a catenated DNA […] Show less (-) |
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TG4120 |
TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of… Show more (+) TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive […] Show less (-) |
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TG4170 |
Ellipticine is a topoisomerase II poison that induces topo II dependent cleavages |
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TG4140 |
TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of… Show more (+) TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II. For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive […] Show less (-) |
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TG4160 |
Genistein is an isoflavone and an inhibitor of tyrosine protein kinases. It has been reported to induce topo II dependent cleavages (Biochem. Pharmacol. 39:737-740 [1990]). TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II (Note, Genistein may also inhibit topoisomerase I.) For in vitro cleavage… Show more (+) Genistein is an isoflavone and an inhibitor of tyrosine protein kinases. It has been reported to induce topo II dependent cleavages (Biochem. Pharmacol. 39:737-740 [1990]). TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II (Note, Genistein may also inhibit topoisomerase I.) For in vitro cleavage detection, we recom- mend that the drug be […] Show less (-) |
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TG4200 |
HU-331 is a cannabinoid-based quinone with potential anticancer activity. It has anti-topo IIa activity as a catalytic inhibitor rather than a poison (such as etoposide). |
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TG4101 |
Irinotecan (CPT-11) Description Irinotecan (also known as CPT-11) is a water soluble, semi-synthetic analogue of 20(S)camptothecin (CPT) with promising activity against a range of tumour types. Irinotecan causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single… Show more (+) Irinotecan (CPT-11) Description Irinotecan (also known as CPT-11) is a water soluble, semi-synthetic analogue of 20(S)camptothecin (CPT) with promising activity against a range of tumour types. Irinotecan causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. [Clin Exp […] Show less (-) |
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TG2013 |
Kinetoplast DNA (kDNA) is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I. Thus, kDNA works well to quantify type II activity in… Show more (+) Kinetoplast DNA (kDNA) is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms. Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I. Thus, kDNA works well to quantify type II activity in crude cell extracts, which are frequently overloaded […] Show less (-) |
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TG2018-1 |
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can… Show more (+) Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can be avoided by using a catenated DNA […] Show less (-) |
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TG2035 |
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Relaxed pHOT-1 Plasmid DNA is the ideal substrate for use assaying the supercoiling activity of DNA gyrase.
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TG2030 |
This plasmid is a perfect substrate for assaying any topoisomerase. pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers. Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase. Supercoiled pHOT-1 is… Show more (+) This plasmid is a perfect substrate for assaying any topoisomerase. pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers. Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase. Supercoiled pHOT-1 is a scaled-down derivative of pBR322 and optimized for higher […] Show less (-) |
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TG4100 |
Hycamtin (topotecan hydrochloride) is a semi-synthetic derivative of camptothecin and is an anti-tumor drug that targets topo I and is a topo I poison (induces cleavage complexes). |
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