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Topo Related Drugs & Assay Reagents

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TG4103
10-Hydroxy Camptothecin has shown promising activity against a range of tumour types. This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. TopoGEN provides this reagent as a control poison/inhibitor of…
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10-Hydroxy Camptothecin has shown promising activity against a range of tumour types. This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I. For in vitro […]
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TG4104
9-Amino-20-(R,S)-camptothecin (also known as 9-amino campthecin) is an analogue of camptothecin.  Like other CPT congeners, it shows some promising activity against a range of tumor types.  This compound causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA…
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9-Amino-20-(R,S)-camptothecin (also known as 9-amino campthecin) is an analogue of camptothecin.  Like other CPT congeners, it shows some promising activity against a range of tumor types.  This compound causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks.  TopoGEN […]
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TG4102
20(S)camptothecin has shown promising activity against a range of tumour types.  This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks.  TopoGEN provides this reagent as a control poison/inhibitor…
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20(S)camptothecin has shown promising activity against a range of tumour types.  This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks.  TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I.  For in vitro cleavage […]
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TG4150
TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of…
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TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive […]
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TG4110
Camptothecin is a topo I poison that both inhibits enzyme activity and stabilizes formation of topo I cleavage complexes (Hsiang et al J. Biol. Chem. 260:14873-14878, 1985).  TopoGEN provides this reagent as a control inhibitor/poison of topoisomerase I.  For in vitro cleavage detection, we recommend that the drug…
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Camptothecin is a topo I poison that both inhibits enzyme activity and stabilizes formation of topo I cleavage complexes (Hsiang et al J. Biol. Chem. 260:14873-14878, 1985).  TopoGEN provides this reagent as a control inhibitor/poison of topoisomerase I.  For in vitro cleavage detection, we recommend that the drug be tested in the range 100 uM; […]
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TG4180
A control poison/inhibitor of DNA Gyrase and Topo IV.  This is also included in the Topo IV/Gyrase Drug Screening Kits.
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TG4130
TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of…
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TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive […]
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TG2020-1
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions.  Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate.  These problems can…
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Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions.  Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate.  These problems can be avoided by using a catenated DNA […]
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TG4120
TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of…
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TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive […]
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TG4170
Ellipticine is a topoisomerase II poison that induces topo II dependent cleavages
$95.00
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TG4140
TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of…
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TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive […]
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TG4160
Genistein is an isoflavone and an inhibitor of tyrosine protein kinases.  It has been reported to induce topo II dependent cleavages (Biochem. Pharmacol. 39:737-740 [1990]).  TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II (Note, Genistein may also inhibit topoisomerase I.)  For in vitro cleavage…
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Genistein is an isoflavone and an inhibitor of tyrosine protein kinases.  It has been reported to induce topo II dependent cleavages (Biochem. Pharmacol. 39:737-740 [1990]).  TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II (Note, Genistein may also inhibit topoisomerase I.)  For in vitro cleavage detection, we recom- mend that the drug be […]
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TG4200
HU-331 is a cannabinoid-based quinone with potential anticancer activity.  It has anti-topo IIa activity as a catalytic inhibitor rather than a poison (such as etoposide).    
$85.00
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TG4101
Irinotecan (CPT-11) Description Irinotecan (also known as CPT-11) is a water soluble, semi-synthetic analogue of 20(S)camptothecin (CPT) with promising activity against a range of tumour types.  Irinotecan causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single…
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Irinotecan (CPT-11) Description Irinotecan (also known as CPT-11) is a water soluble, semi-synthetic analogue of 20(S)camptothecin (CPT) with promising activity against a range of tumour types.  Irinotecan causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. [Clin Exp […]
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TG2013
kDNA is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms.  Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I.  Thus, kDNA works well to quantify type II activity in crude cell…
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kDNA is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms.  Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I.  Thus, kDNA works well to quantify type II activity in crude cell extracts, which are frequently overloaded with topoisomerase […]
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TG2018-1
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions.  Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate.  These problems can…
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Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions.  Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate.  These problems can be avoided by using a catenated DNA […]
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$135.00
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TG2023-2
20 ug pRYG-DNA-Data-Sheet
$325.00
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TG2035
Relaxed pHOT-1 is suited to assaying the supercoiling activity of DNA gyrase.  It is also useful for assaying alterations in DNA linking number for assessing intercalation.   Relaxed pHOT-1 DNA is prepared using high purity topo I relaxation reactions, followed by inactivation and re-purification of the relaxed product. …
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Relaxed pHOT-1 is suited to assaying the supercoiling activity of DNA gyrase.  It is also useful for assaying alterations in DNA linking number for assessing intercalation.   Relaxed pHOT-1 DNA is prepared using high purity topo I relaxation reactions, followed by inactivation and re-purification of the relaxed product.  TopoGEN staff then verify the relaxation status of […]
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TG2030
This plasmid is a perfect substrate for assaying any topoisomerase.  pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers.  Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase.  Supercoiled pHOT-1 is…
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This plasmid is a perfect substrate for assaying any topoisomerase.  pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers.  Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase.  Supercoiled pHOT-1 is a scaled-down derivative of pBR322 and optimized for higher […]
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TG4100
Hycamtin (topotecan hydrochloride) is a semi-synthetic derivative of camptothecin and is an anti-tumor drug that targets topo I and is a topo I poison (induces cleavage complexes).
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