These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
A new study from Northwestern University and Sonoma State University (Sun et al., 2026, bioRxiv) provides direct physical evidence that mitotic chromosomes aren’t the fully separate units they appear to be under the microscope. Using micropipette manipulation to gently extract chromosomes from living HeLa and mouse fibroblast cells, the researchers found that pulling on a […]
OK, this paper actually has massive implications for topoisomerase drug discovery because it challenges a long-standing assumption about TOP2 poisons. Rather than simply trapping the cleavage complex (as etoposide does), the authors propose that their modified epipodophyllotoxin derivative actively dismantles the TOP2β catalytic apparatus, producing an irreversible, religation-defective complex that leads to persistent DNA double-strand breaks. Topoisomerase […]
