These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
A new study from Northwestern University and Sonoma State University (Sun et al., 2026, bioRxiv) provides direct physical evidence that mitotic chromosomes aren’t the fully separate units they appear to be under the microscope. Using micropipette manipulation to gently extract chromosomes from living HeLa and mouse fibroblast cells, the researchers found that pulling on a […]
A remarkable new study from investigators at the National Institutes of Health has uncovered an unexpected mechanism underlying one of chemotherapy’s most debilitating side effects: cisplatin-induced peripheral neuropathy. Rather than DNA repair simply protecting neurons, the researchers found that DNA repair itself can become toxic when neurons lack sufficient deoxynucleotide (dNTP) pools to complete the repair process. Their […]
A new study published in Nature Structural & Molecular Biology provides important insights into how cells repair DNA damage arising from collapsed replication forks—one of the most significant threats to genome stability and a key driver of cancer, aging, and genetic disease. The authors demonstrate that repair outcomes differ dramatically depending on whether a single replication fork […]
