These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
A groundbreaking new Nature study reveals that cancer cells use specialized genomic “retention elements” to preserve and propagate extrachromosomal circular DNA (ecDNA)—a major engine of oncogene amplification and tumor evolution. These circular ecDNAs, which lack centromeres and segregate randomly, should theoretically be lost at each cell division. Yet this work shows that cancer cells have evolved a mechanism […]
A major Cell paper has uncovered a completely new layer of DNA repair biology: cells use selective autophagy and lysosomes to process and remove Topoisomerase I cleavage complexes (TOP1cc)—the toxic DNA–protein crosslinks created during normal replication and when cells are exposed to TOP1-targeting drugs like camptothecin. According to the Cell article (2024), researchers discovered that: […]
What if the secret to slowing human aging isn’t a mystery at all, but a DNA repair trick stolen from a strange little creature that lives underground? A new Science paper just revealed something jaw-dropping: 👉 Naked mole-rats (also known as the sand puppy 😃) live ~40 years because their DNA repair machinery is turbo-charged […]
They Said This Breast Cancer Was Untouchable. Now It’s 70% Gone! This is why TopoGEN is a key player, providing tools that allow anti-cancer drug discovery and facilitate such important findings. Scientists have designed a new “smart bomb” drug, DM1, that obliterates the deadliest form of breast cancer—triple-negative breast cancer—by hitting two critical targets at […]
Are you working with agents that target Human Top1? This short video discusses the use and relevance of Our Topoisomerase I Drug Screening Kit. Learn how to quickly identify novel inhibitors of Human Top1.
What does TopoGEN do, exactly? We’re so glad you asked! Check out this video for the TopoGEN backstory, a glimpse at our lab space, and in particular Dr. Muller’s vision for attracting more like-minded tech to Buena Vista, Colorado. Huge shout out to the Central Colorado SBDC for putting this together!
TopoGEN products (Top2a) were used in the following study published in Nature. This is a very exciting and powerful result suggesting novel inroads to addressing the problem of cardiotoxicity. In this work, authors develop obex inhibitors that target a distinct binding pocket in the ATPase domain of Topoisomerase II. They demonstrate how Topobexin, a Topoisomerase […]
Transfection-based Homologous Recombination (HR) and Non-homologous End Joining (NHEJ) kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are: It uses GFP readout to measure the efficiency of repair. There is zero background when repair is […]
Contract Screening Services Exciting News from TopoGEN, Inc. 🚀 We are thrilled to announce the launch of our groundbreaking screening tool, the **IQ Screen** This novel and affordable tool is designed specifically for researchers seeking a rapid go/no-go decision on which compounds to advance as anti-topoisomerase agents. IQ stands for “Insight Quick” and is ideal for […]
Cell Context DNA Repair Kits Are you working on DNA-damage and repair? Would you be interested in a pre-validated cell-context system to analyze HR or NHEJ by single cell imaging or quantifying DNA repair by simple fluorescence? TopoGEN, Inc. has developed a suite of cell lines that will allow you to study specific DNA repair […]
