These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
It uses GFP readout to measure the efficiency of repair.
There is zero background when repair is blocked or inhibited
The reporters for HR or NHEJ can be installed in virtually any cell line that can be transiently transfect
It is rapid and robust
It is ideal for drug screens to find novel inhibitors of HR/NHEJ
The kits are highly specific and can only report HR or NHEJ
Readout GFP can be done by FACS or Imaging
Entire pathway can be followed by live imaging in viable cells
Ideal to track repair kinetics at the single cell level
DNA Repair pathways in animal cells can be divided into two main categories: HR and NHEJ. HR or homologous recombination is a minor pathway but very important in protecting cells from genotoxicity. The process has two key requirements as well: a homologous sequence, usually available after DNA replication when the genome is 4N, and S-phase. A specific reporter based assay for HR can be very beneficial for anti-cancer drug discovery projects, learning more about the process of DNA HR repair and establishing intersecting pathways and druggable pathway targets. A cellular context for HR improves the physiological relevance of the results.
This…
Custom Cell Based Screening Kit for NHEJ contains reagents necessary to create a complete NHEJ reporter system in any animal cell line. It includes the NHEJ-GFP reporter with a selectable marker and a DS DNA cleavage nuclease to initiate DNA repair.
HR DNA Repair Reporter Kit by Plasmid Transfection Assay designed to allow researchers to study Homologous Recombination (HR) in a cell line of their choosing. The kit uses GFP as an in vivo readout for the HR pathway and includes proper controls to set the system up in your own lab. NEW: we are now offering an affordable trial size (50ug each of 3 plasmids in the Kit) to allow you to test the power of our new cell-based kits. We offer academic and for-profit pricing, please contact us for a quote.
This is a cell-based reporter kit that is hosted by a human neuroblastoma cell line (SH-SY5Y) that can grow continuously in vitro and will terminally differentiate. This provides the opportunity of analyzing NHEJ in growing or terminally differentiated cells in a neuronal lineage.
This is a cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in vivo readout for the HR pathway and Crispr-CAS gRNA plasmid that drives a DS DNA break in Cassette 1. This DS break induces HR in a mutated GFP reporter gene, which recombines with Cassette 2 containing WT GFP leading to GFP+ cells after completing HR.
This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in situ readout for the HR pathway.
DNA Repair pathways in animal cells can be divided into two main categories: HR and NHEJ. HR or homologous recombination is a minor pathway but very important in protecting cells from genotoxicity. HR needs a homologous sequence and is limited to S-phase. A specific reporter based assay for HR can be very beneficial for drug discovery projects, learning more about the process of DNA HR repair and establishing intersecting pathways and druggable pathway targets.
This is the first HR reporter that has been engineered into a neuronally derived cell line. The SH-SY5Y cell line (SH) is a subclone of a bone…
This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and other genes) that affect or impact NHEJ repair. The kit uses GFP as an in situ readout for NHEJ activity in a cell context.
This is a cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in vivo readout for the HR pathway and a plasmid expression vector containing the mega-endonuclease I-Sce1. The HR reporter plasmid has been engineered into a mouse 3T3 cell line which is ideal for cell cycle analyses.
TopoGEN scientists have created a novel, cell based kit to follow NHEJ by simply assaying for the presence of GFP positive cells. The CN1.4 cell line is a clone derived from mouse embryonic day 13 neuronal cultures. The line was transformed using the temperature-sensitive SV-40 Large T-antigen The kit uses GFP as an in situ readout for NHEJ activity in a cell context.
This is the first HR reporter that has been engineered into a neuronally derived mouse cell line model. The CN1.4 cell line is a clone derived from mouse embryonic day 13 neuronal cultures. The line was transformed using the temperature-sensitive SV-40 Large T-antigen. The cell line expresses numerous neuronal markers, including neurofilaments, glutamic acid decarboxylase 67, neuron-specific enolase, and the BG21 isoform of the myelin basic protein gene.
This is a Mouse 3T3 cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and other genes) that affect or impact NHEJ repair. The kit uses GFP as an in situ readout for NHEJ activity in a cell context.
This is a plasmid transfection based reporter system designed to allow the customer to screen or interrogate any agent (drug or protein) that targets total NHEJ in the context of a live cell. The reporter plasmid in this kit reports on total NHEJ processes, which encompasses both classic and alternate pathways. It will work in most of all cell lines that can be transfected. The kit uses GFP as an in vivo readout for NHEJ and includes two Plasmids that are validated as high quality, transfection-grade DNA (see Figures 1 & 2).
NEW: we are now offering an affordable trial size…
When you become our customer, we become your partner in research. We review your data and offer valuable input. Feel free to contact us for further information about how we can assist your topoisomerase research endeavors.
