A newly published study in Scientific Reports describes the identification and characterization of alternariol monomethyl ether (AME), a fungal secondary metabolite isolated from Alternaria alternata, with potent antiproliferative activity linked to inhibition of both DNA Topoisomerase I and II. Importantly, the investigators utilized TopoGEN’s plasmid relaxation and kDNA decatenation assay systems to quantify enzyme inhibition kinetics and demonstrate […]

A new Phytomedicine (2026) study caught my attention—not because it shows anti-melanoma activity (we’ve seen that before), but because it begins to unpack how a plant-derived formulation enhances the efficacy of temozolomide (TMZ).

A newly published study in the Journal of Molecular Biology (2026) provides important mechanistic insight into how tyrosyl-DNA phosphodiesterase I (Tdp1) processes topoisomerase-mediated DNA damage—extending its functional relevance beyond its classical role in Top1 repair and into the Top2 space.