These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
A new Nature Communications study is reshaping how scientists think about the origins of cancer-causing mutations. The research focuses on a DNA-managing enzyme called TOP2B (topoisomerase IIβ)—an essential “stress-relief” protein that helps untangle DNA so genes can be switched on. Until now, TOP2B was mostly viewed as a helpful regulator of transcription. But this new […]
A groundbreaking new Nature study reveals that cancer cells use specialized genomic “retention elements” to preserve and propagate extrachromosomal circular DNA (ecDNA)—a major engine of oncogene amplification and tumor evolution. These circular ecDNAs, which lack centromeres and segregate randomly, should theoretically be lost at each cell division. Yet this work shows that cancer cells have evolved a mechanism […]
A major Cell paper has uncovered a completely new layer of DNA repair biology: cells use selective autophagy and lysosomes to process and remove Topoisomerase I cleavage complexes (TOP1cc)—the toxic DNA–protein crosslinks created during normal replication and when cells are exposed to TOP1-targeting drugs like camptothecin. According to the Cell article (2024), researchers discovered that: […]
What if the secret to slowing human aging isn’t a mystery at all, but a DNA repair trick stolen from a strange little creature that lives underground? A new Science paper just revealed something jaw-dropping: 👉 Naked mole-rats (also known as the sand puppy 😃) live ~40 years because their DNA repair machinery is turbo-charged […]
