These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
A new publication in the European Journal of Medicinal Chemistry reports a surprising and important discovery about how certain gold(III) compounds interact with human topoisomerase IIα (Top2α)—and the work was made possible using TopoGEN products.
A new Nature Communications study is reshaping how scientists think about the origins of cancer-causing mutations. The research focuses on a DNA-managing enzyme called TOP2B (topoisomerase IIβ)—an essential “stress-relief” protein that helps untangle DNA so genes can be switched on. Until now, TOP2B was mostly viewed as a helpful regulator of transcription. But this new […]
A groundbreaking new Nature study reveals that cancer cells use specialized genomic “retention elements” to preserve and propagate extrachromosomal circular DNA (ecDNA)—a major engine of oncogene amplification and tumor evolution. These circular ecDNAs, which lack centromeres and segregate randomly, should theoretically be lost at each cell division. Yet this work shows that cancer cells have evolved a mechanism […]
A major Cell paper has uncovered a completely new layer of DNA repair biology: cells use selective autophagy and lysosomes to process and remove Topoisomerase I cleavage complexes (TOP1cc)—the toxic DNA–protein crosslinks created during normal replication and when cells are exposed to TOP1-targeting drugs like camptothecin. According to the Cell article (2024), researchers discovered that: […]
What if the secret to slowing human aging isn’t a mystery at all, but a DNA repair trick stolen from a strange little creature that lives underground? A new Science paper just revealed something jaw-dropping: 👉 Naked mole-rats (also known as the sand puppy 😃) live ~40 years because their DNA repair machinery is turbo-charged […]
They Said This Breast Cancer Was Untouchable. Now It’s 70% Gone! This is why TopoGEN is a key player, providing tools that allow anti-cancer drug discovery and facilitate such important findings. Scientists have designed a new “smart bomb” drug, DM1, that obliterates the deadliest form of breast cancer—triple-negative breast cancer—by hitting two critical targets at […]
Are you working with agents that target Human Top1? This short video discusses the use and relevance of Our Topoisomerase I Drug Screening Kit. Learn how to quickly identify novel inhibitors of Human Top1.
What does TopoGEN do, exactly? We’re so glad you asked! Check out this video for the TopoGEN backstory, a glimpse at our lab space, and in particular Dr. Muller’s vision for attracting more like-minded tech to Buena Vista, Colorado. Huge shout out to the Central Colorado SBDC for putting this together!
TopoGEN products (Top2a) were used in the following study published in Nature. This is a very exciting and powerful result suggesting novel inroads to addressing the problem of cardiotoxicity. In this work, authors develop obex inhibitors that target a distinct binding pocket in the ATPase domain of Topoisomerase II. They demonstrate how Topobexin, a Topoisomerase […]
