These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
$105.00
Topo IV Assay Buffer is supplied as a 5x Stock solution in two parts that must be mixed prior to use:
These two buffers must be mixed to prepare a final 5x complete Topo IV Assay Buffer (A+B) as follows: add 0.1 volume of Buffer B to 1.0 volume Buffer A. Prepare only as much of the complete Topo IV Assay Buffer (A+B) as needed for each day or set of experiments. Note that this buffer should be kept on ice and used the same day, then discarded.
If you want to order this product, please contact us.
Topo IV Assay Buffer is supplied as a 5x Stock solution in two parts that must be mixed prior to use:
These two buffers must be mixed to prepare a final 5x complete Topo IV Assay Buffer (A+B) as follows: add 0.1 volume of Buffer B to 1.0 volume Buffer A. Prepare only as much of the complete Topo IV Assay Buffer (A+B) as needed for each day or set of experiments. Note that this buffer should be kept on ice and used the same day, then discarded.
Quality Control Analysis:
The complete Topo IV Assay Buffer (A+B) is optimized and tested for decatenation and relaxation activity of e. coli topo IV. The complete buffer should be made fresh for each experiment.
The two-part buffer is shipped on blue ice (or on dry ice if included with enzyme) and should be stored at -20°C. The complete Topo IV Assay Buffer (A+B) is stable for 24 hours at 4°C.
If you require additional information about this product, please contact us at: support@topogen.com
