These HR/NHEJ kits are cell based, meaning they have the HR or NHEJ GFP reporters pre-installed. A single plasmid (I-Sce1) is transfected to activate repair. Please see this table for a summary and comparison/application of each kit.
Homologous GFP Repair Systems
HeLa Cell HR Kit (I-Sce1 Based)
HeLa Cell HR Kit (CRISPR-CAS Based)
Human Neuroblastoma Cell Line SH-SY5Y using (I-Sce1 transfection)
Mouse 3T3 Cell Line using I-Sce1 transfection
Mouse Neuronal Cell Line using I-Sce1 transfection
Custom construction Kit to make your own HR Cell Reporter
NHEJ GFP Reporter
Systems
Transfection-based HR and NHEJ kits use transient transfections to make a pool of cells containing the DNA reporters to allow the investigator to interrogate the 2 major DNA repair pathways. The advantages are:
A new Phytomedicine (2026) study caught my attention—not because it shows anti-melanoma activity (we’ve seen that before), but because it begins to unpack how a plant-derived formulation enhances the efficacy of temozolomide (TMZ).
A newly published study in the Journal of Molecular Biology (2026) provides important mechanistic insight into how tyrosyl-DNA phosphodiesterase I (Tdp1) processes topoisomerase-mediated DNA damage—extending its functional relevance beyond its classical role in Top1 repair and into the Top2 space.
