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TG2020-1
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions.  Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate.  These problems can…
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Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions.  Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate.  These problems can be avoided by using a catenated DNA […]
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$135.00
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TG2013
Kinetoplast DNA (kDNA) is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms.  Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I.  Thus, kDNA works well to quantify type II activity in…
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Kinetoplast DNA (kDNA) is the ideal substrate for topoisomerase II assays because it is specific for type II reaction mechanisms.  Researchers can even assay for a type II enzyme in the presence of large excess of topoisomerase I.  Thus, kDNA works well to quantify type II activity in crude cell extracts, which are frequently overloaded […]
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TG2018-1
Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions.  Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate.  These problems can…
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Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions.  Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate.  These problems can be avoided by using a catenated DNA […]
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$135.00
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TG2023-2
20 ug pRYG-DNA-Data-Sheet
$325.00
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TG2035
Relaxed pHOT-1 DNA is suited to assaying the supercoiling activity of DNA gyrase.  It is also useful for assaying alterations in DNA linking number for assessing intercalation.    
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TG2030
This plasmid is a perfect substrate for assaying any topoisomerase.  pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers.  Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase.  Supercoiled pHOT-1 is…
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This plasmid is a perfect substrate for assaying any topoisomerase.  pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers.  Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase.  Supercoiled pHOT-1 is a scaled-down derivative of pBR322 and optimized for higher […]
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