Human Topoisomerase I

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SKU: TG2005HRC Category: Tags: , , ,

High purity, catalytically active Top1 Enzyme overexpressed in the baculovirus system and purified to single band homogeneity.  These preparations are cheaper than native topo I made from human tissue and offer excellent purity and high specific activity.  In addition, the 100 kDa form of  recombinant topo I is stable and is less prone to proteolysis, relative to the native protein from human cells.

Included Materials:
10X assay buffer and dilution buffer is included.  These may be purchased separately as TGS 10X Topo I Assay Buffer (TG4095).

The enzyme is shipped on dry ice and should be stored at -20°C. It has a life-time of about 6 months when stored under optimal conditions. Repeat freeze/thaw cycles will accelerate loss.

  • Reviews and Citations:
  • Yoshizaki A, Yanaba K, Ogawa A, Asano Y, Kadono T, Sato S: Immunization With DNA Topoisomerase I and Freund’s Complete Adjuvant Induces Skin and Lung Fibrosis and Autoimmunity via Interleukin-6 Signaling.  Arthritis and Rheumatism 2011, 63: 3575-3585.  doi: 10.1002/art.30539.
  • Zhang L, Sullivan P, Suyama J, et. al: Epidermal growth factor-induced heparanase nucleolar localization augments DNA topoisomerase I activity in brain metastatic breast cancer.  Molecular Cancer Research 2010, 8: 278-290. doi: 10.1158/1541-7786.MCR-09-0375

Recombinant-Human-Topoisomerase-I-Enzyme-Data-Sheet

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