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Name
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Eukaryotic Topoisomerase II Drug Screening Kit 100 Assays
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Price
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$269.00
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SKU/Part Number
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1009-1
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Extended Description
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Topo II inhibitors have been shown to possess effective antibacterial, antiviral and anticancer activity. Identification of topo II inhibitors and antagonists requires tractable biochemical assays employing purified enzyme. The TopoGEN Drug Screening Kit contains the reagents necessary to screen for agents that affect enzymatic activity. To allow the customer maximum flexibility, purified topoisomerase II may be purchased separately from the kit (Catalog number 2000H-1 and 2000H-2). This kit will allow detection of two classes of topoisomerase inhibitors: those that stimulate formation of cleavable complexes and those that antagonize topo II action on the DNA. Also included in this kit is detailed documentation on design and interpretation of inhibition data. Known inhibitors are included as controls. The DNA substrate (pHOT1) included in this assay is ideal for these studies because it contains a single, high affinity topo II cleavage site (note that topo II is not particularly sequence specific). The assay system is based upon evaluating the formation of DNA cleavage products which may be nicked, open circular DNA or linear DNA. The products are then resolved by ethidium bromide gel electrophoresis as described in the kit.
Kit Contents (representative of 100 assay kit size):
-Supercoiled DNA [pHOT1 DNA] 25 ug
-Markers are linear pHOT1 DNA and open circular DNA
-10X Topoisomerase II assay buffer components*
*NEW: To improve stability, the 10x Topo II Assay buffer is provided as two components: Buffer A (no ATP) and Buffer B (ATP). The Complete 10x Assay buffer is made fresh each time by combining Buffer A and B.
-Sodium dodecyl sulfate (10%)
-10X Gel loading buffer (bromophenol blue, glycerol)
-Control inhibitor, Etoposide
-Detailed instruction manual
-Proteinase K is now included with this kit
Note: Enzyme is sold separately.
Shipping/Storage Conditions:
This kit is shipped at ambient temperature; storage is at -20 C (long term).
Related Products: p170 Human Topo II (Cat. No. 2000H), Doxorubicin (Cat. No. 4120), Daunomycin (Cat. No. 4130), Etoposide (Cat. No. 4140), m-AMSA (Cat. No. 4150), Genistein (Cat. No. 4160), Ellipticine (Cat. No. 4170)
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