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Name
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Tdp1 Assay Kit 250 Assays
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Price
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$349.00
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SKU/Part Number
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1004-2
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Extended Description
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This kit contains all reagents for routine detection of Tdp1 activity using the tyrosine release assay from the substrate Oligo-Tyr. The assay is highly specific for Tdp1.
Summary: The kit contains reagents and protocols for assaying Tyrosyl DNA Phosphodiesterase (Tdp1). A procedure for making suitable cell extracts is described at the end of this manual.
Related Products: Human Tdp1 (Cat. No. 2004H)
Kit Contents: Volumes are given for the 100 assay kit size. For the 250 assay kit size, multiply volumes by 2.5 fold. Note that this kit does not include Tyrosyl DNA Phosphodiesterase enzyme, which is available from TopoGEN (Cat #2004). We recommend reconstructing the assay using the purified Tdp1 to ensure that the assay is optimized and working well.
-Oligo-Tyr substrate (10 ul at 0.8 ug/ul in TE buffer [10 mM Tris, pH 7.5, 1 mM EDTA]). This substrate should be end labeled with T4 polynucleotide kinase and g-[32P]- ATP as described in the kit protocol
-5x Tdp1 assay buffer (300 ul): 5x buffer contains 100 mM Tris-HCl (pH 7.5), 500 mM KCl, 50 mM EDTA, 5mM dithiothreitol (DTT)
-2x Stop Buffer (gel loading buffer) (200 ul): 96% formamide, 20 mM EDTA, 0.03% xylene cyanol, 0.03% bromophenol blue
Shipping and Recommended Storage Conditions:
This kit is shipped on wet ice. The buffers and oligo can be stored at 4° C (short term storage) or at -20° C (long term). The components in this kit are not sensitive to freezing and thawing.
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