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Name
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Eukaryotic Topoisomerase I Assay Kit 250 Assays
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Price
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$349.00
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SKU/Part Number
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1015-2
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Extended Description
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This assay kit contains reagents for routine detection of type I topoisomerase activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts. A detailed manual of instruction is included that describes how to run the gels to maximally resolve topoisomers for analysis of DNA unwinding.
Kit Contents: (representative for 100 assay kit size)
-Supercoiled (form I) plasmid substrate DNA (25 µg in 100 µl TE buffer, or 0.25 µg/ul)
-Relaxed plasmid substrate DNA marker, relaxed by topo I (50 ul at 0.05 µg/ml in gel loading buffer)
-10X Topo I assay buffer (300 µl)
-5X Stop buffer/gel loading dye (600 µl)
-Detailed instruction manual
-Sample data and documentation of all controls
Shipping and Recommended Storage Conditions:
This kit is shipped at ambient temperature and should be stored at 4 C.
Related Products: 10X Topo I Assay Buffer (Cat. No. 4010), pHOT1 Supercoiled DNA (Cat. No. 2030), pHOT1 Relaxed DNA (Cat. No. 2035)
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