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Name
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Bacterial DNA Gyrase (E. coli) 2000 Units
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Price
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$1199.00
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SKU/Part Number
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2000G-7
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Extended Description
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Highly purified E. coli gyrase is offered as a holoenzyme (contains both A,B subunits). This is an excellent reagent for supercoiling plasmids in vitro or for novel drug screens.
Quality Control Tests:
Nuclease contamination: assayed by testing for linear KDNA and linear plasmid DNA formation. Incubation of 1 µg of catenated KDNA or supercoiled pUC19 DNA for 4 hrs. at 37 C (under gyrase assay conditions and with or without ATP).
Assay Conditions:
Supercoiling assays are carried out using relaxed pBR-322 DNA under conditions specified in the protocol sheets provided with the product. We also perform assays using kDNA as substrate. For details on this decatenation/supercoiling assay please refer to our technical literature. One unit of gyrase activity will supercoil 0.25 ug pBR-322 DNA in 30 min at 37 C.
A 5X assay buffer and dilution buffer is included with the enzyme.
Storage and Shipping Conditions:
The enzyme is shipped on dry ice and should be stored at -70 C. We also recommend that the enzyme be aliquoted after the first thaw (repeated rounds of freeze/thaw may cause loss of activity); the enzyme activity is stable for 1-3 days on ice.
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