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Name
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Human Tdp1 250 Units
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Price
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$269.00
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SKU/Part Number
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2004H-1
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Extended Description
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TopoGEN provides purified Human Tyrosyl DNA Phosphodiesterase (Tdp1) purified to homogeneity to a single 68 kDa band on SDS-PAGE. Tdp1 is catalytically active and will remove a terminal 3’ phosphotyrosyl group from a specifically designed oligo target site (oligo-Tyr). Additionally, TopoGEN scientists have demonstrated that Tdp1 will release topo I covalently attached to genomic DNA isolated from cells treated with camptothecin using TopoGEN’s In Vivo Link Kit. Tdp1 is thought to be important in repair of topo/DNA adducts that form following topo-active drug therapy; therefore, there is considerable interest in identifying drugs that target Tdp1. Such drugs may work well in combination with topo targeting strategies.
Unit Defintion:
One unit of Tdp1 can release the terminal Tyrosine from 20 ng of substrate (oligo-Tyr) in 30 min at 37 C.
Tdp1 Assays:
Biochemical assays for Tdp1 can be carried out using an oligo nucleotide with a 3’ terminal tyrosine residue. Release of tyrosine from a 5’ 32P end labeled oligo is easily measured using a denaturing polyacrylamide gel or DNA sequencing gels. TopoGEN Provides a kit for Tdp1 assay that has key reagents and controls/protocols for setting up a complete assay for Tdp1; see Catalog # 1004.
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