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Name
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Relaxed pRYG DNA Marker 10ug
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Price
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$109.00
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SKU/Part Number
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2025-1
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Extended Description
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pRYG DNA: a plasmid containing multiple "clusters" of topo II cleavage sites.
pRYG is a pUC19 derivative in which the 206 bp SphI-NarI fragment of pUC19 was replaced with a 245 bp SphI-AccI fragment upstream of the human adult beta-globin gene promoter. The 245 bp fragment contains a 54 bp alternating repeat of purine/pyrimidine sequence that constitutes a strong eukaryotic topo II cleavage site. Cleavages within the repeat are readily detected in the absence of topo II inhibitors such as m-AMSA or VM-26. The sequence of the plasmid is given in Table 2 of Spitzner et al.
Shipping and Storage:
The plasmid is shipped at ambient temperature and should be stored at 4 C.
REFERENCE:
For detailed description of pRYG, its reactivity with Topo II and the sequence, see Spitzner et al., Nuc. Acid. Res. 18: 1-11 (1990)
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